Effects of the KIF2C neck peptide on microtubules: lateral disintegration of microtubules and β-structure formation
Article first published online: 7 MAR 2013
© 2013 The Authors Journal compilation © 2013 FEBS
Volume 280, Issue 7, pages 1681–1692, April 2013
How to Cite
Shimizu, Y., Shimizu, T., Nara, M., Kikumoto, M., Kojima, H. and Morii, H. (2013), Effects of the KIF2C neck peptide on microtubules: lateral disintegration of microtubules and β-structure formation. FEBS Journal, 280: 1681–1692. doi: 10.1111/febs.12182
- Issue published online: 27 MAR 2013
- Article first published online: 7 MAR 2013
- Accepted manuscript online: 9 FEB 2013 01:50AM EST
- Manuscript Accepted: 7 FEB 2013
- Manuscript Revised: 23 JAN 2013
- Manuscript Received: 28 DEC 2012
Fig. S1. CD spectra of neck region peptides and microtubules.
Fig. S2. IR spectra of microtubules with non-labeled KF501 peptide and an evaluation of spectral changes by 13C labeling.
Fig. S3. IR difference spectra under maximally perturbed normalization conditions compared with those under non-perturbed conditions.
Table S1. Fitting parameters of an IR spectrum of a mixture of microtubules and KF501 peptide resolved by multiple Gaussian curves.
Table S2. Fitting parameters of difference IR spectra between the original spectrum of a peptide with a 13C label at certain main-chain amides and that without a label.
Table S3. Fitting parameters of difference IR spectra under maximally perturbed normalization conditions.
Doc. S1. Size information for the ring structures formed from microtubules and the neck region peptides.
Doc. S2. IR spectroscopic analysis on the complex of microtubules and the neck region peptides.
Doc. S3. Evaluation for secondary structures of the peptide in interaction with microtubules.
Doc. S4. Scrutinization for normalization of IR spectra.
Doc. S5. Synthesis of the peptides from the neck region of KIF2C.
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