• 1
    World Health Organization (2004) WHO Guidelines for the Global Surveillance of Severe Acute Respiratory Syndrome (SARS). World Health Organization, Geneva, Switzerland.
  • 2
    Ziebuhr J (2004) Molecular biology of severe acute respiratory syndrome coronavirus. Curr Opin Microbiol 7, 412419.
  • 3
    Yang H, Yang M, Ding Y, Liu Y, Lou Z, Zhou Z, Sun L, Mo L, Ye S, Pang H et al. (2003) The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc Natl Acad Sci USA 100, 1319013195.
  • 4
    Kuo C-J, Chi Y-H, Hsu JT-A & Liang P-H (2004) Characterization of SARS main protease and inhibitor assay using a fluorogenic substrate. Biochem Biophys Res Commun 318, 862867.
  • 5
    Xue X, Yang H, Shen W, Zhao Q, Li J, Yang K, Chen C, Jin Y, Bartlam M & Rao Z (2007) Production of authentic SARS-CoV MPro with enhanced activity: application as a novel tag-cleavage endopeptidase for protein overproduction. J Mol Biol 366, 965975.
  • 6
    Verschueren KHG, Pumpor K, Anemüller S, Chen S, Mesters JR & Hilgenfeld R (2008) A structural view of the inactivation of the SARS coronavirus main proteinase by benzotriazole esters. Chem Biol 15, 597606.
  • 7
    Hsu M-F, Kuo C-J, Chang K-T, Chang H-C, Chou C-C, Ko T-P, Shr H-L, Chang G-G & Wang AH-J (2005) Mechanism of the maturation process of SARS-CoV 3CL protease. J Biol Chem 280, 3125731266.
  • 8
    Thiel V, Ivanov KA, Putic A, Hertzig T, Schelle B, Bayer S, Weissbrich B, Snijder EJ, Rabenau H, Doerr HW et al. (2003) Mechanisms and enzymes involved in SARS coronavirus genome expression. J Gen Virol 84, 23052315.
  • 9
    Inoue S & Tsuji FI (1994) Aequorea green fluorescent protein. Expression of the gene and fluorescent characteristics of the recombinant protein. FEBS Lett 341, 277280.
  • 10
    Fan K, Wei P, Feng Q, Chen C, Hung C, Ma L, Lai B, Pei J, Liu Y, Chen J et al. (2004) Biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3C-like proteinase. J Biol Chem 279, 16371642.
  • 11
    Chuck C-P, Chong L-T, Chen C, Chow H-F, Wan DC-C & Wong K-B (2010) Profiling of substrate specificity of SARS-CoV 3CLpro. PLoS One 5, e13197.
  • 12
    Li C, Qi Y, Teng X, Yang Z, Wei P, Zhang C, Tan L, Zhou L, Liu Y & Lai L (2010) Maturation mechanism of severe acute respiratory syndrome (SARS) coronavirus 3C-like protease. J Biol Chem 285, 2813428140.
  • 13
    Chen S, Hu T, Zhang J, Chen J, Chen K, Ding J, Jiang H & Shen X (2008) Mutation of Gly-11 on the dimer interface results in the complete crystallographic dimer dissociation of severe acute respiratory syndrome coronavirus 3C-like protease. Crystal structure with molecular dynamics simulations. J Biol Chem 283, 554564.
  • 14
    Shi J, Sivaraman J & Song J (2008) Mechanism for controlling the dimer–monomer switch and coupling dimerization to catalysis of the severe acute respiratory syndrome coronavirus 3C-like protease. J Virol 82, 46204629.
  • 15
    Hu T, Zhang Y, Li L, Wang K, Chen S, Chen J, Ding J, Jiang H & Shen X (2009) Two adjacent mutations on the dimer interface of SARS coronavirus 3C-like protease cause different conformational changes in crystal structure. Virology 388, 324334.
  • 16
    Chen S, Jonas F, Shen C & Hilgenfeld R (2010) Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode. Protein Cell 1, 5974.
  • 17
    Oostra M, te Lintelo EG, Deijs M, Verheije MH, Rottier PJM & de Haan CAM (2007) Localization and membrane topology of coronavirus nonstructural protein 4: involvement of the early secretory pathway in replication. J Virol 81, 1232312336.
  • 18
    Oostra M, Hagemeijer MC, van Gent M, Bekker CPJ, te Lintelo EG, Rottier PJM & de Haan CAM (2008) Topology and membrane anchoring of the coronavirus replication complex: not all hydrophobic domains of nsp3 and nsp6 are membrane spanning. J Virol 82, 1239212405.
  • 19
    Kigawa T, Yabuki T, Matsuda N, Matsuda T, Nakajima R, Tanaka A & Yokoyama S (2004) Preparation of Escherichia coli cell extract for highly productive cell-free protein expression. J Struct Funct Genomics 5, 6368.
  • 20
    Kigawa T, Yabuki T, Yoshida Y, Tsutsui M, Ito Y, Shibata T & Yokoyama S (1999) Cell-free production and stable-isotope labeling of milligram quantities of proteins. FEBS Lett 442, 1519.
  • 21
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680685.