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Fig. S1. Representative total ion chromatograms and extracted ion chromatograms for HS Δ4,5-unsaturated disaccharides.

Fig. S2. Representative SEC-MS data showing total ion chromatograms and extracted ion chromatograms for CS disaccharides and GalNAcSO3.

Fig. S3. Relative ion abundances of Δ4,5-unsaturated HS disaccharides from recombinant syndecan-4 and HS from bovine kidney.

Fig. S4. Summary of the number of sulfate groups per 100 HS disaccharides from four donors.

Table S1. The cell number percentage of purified B cells, T cells, NK cells, and monocytes in PBMCs of four donors.

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