Fig. S1. Fluorescence emission spectra (500–660 nm) at 25 °C for CaM-D and CaM-DA (excitation at 480 nm) in 2 mm Tris/HCl pH 7.5 without and with 150 mm KCl.

Fig. S2. GuHCl denaturation of Ca4-CaM (CaM in Tris/HCl, pH 7.5, with 10 mm CaCl2) as monitored by the ellipticity at 222 nm.

Fig. S3. Binding of smMLCK-peptide to CaM-DA.

Fig. S4. SDS polyacrylamide gel electrophoresis.

Fig. S5. Peak deconvolution from absorbance spectra of FRET-CaM.

Table S1. Distance between the α-carbons of residues 17 and 117 in available NMR and X-ray structures of calmodulin.

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