Inhibition of vacuolar ATPase attenuates the TRAIL-induced activation of caspase-8 and modulates the trafficking of TRAIL receptosomes
Version of Record online: 18 JUN 2013
© 2013 FEBS
The FEBS Journal
Volume 280, Issue 14, pages 3436–3450, July 2013
How to Cite
Horova, V., Hradilova, N., Jelinkova, I., Koc, M., Svadlenka, J., Brazina, J., Klima, M., Slavik, J., Hyrslova Vaculova, A. and Andera, L. (2013), Inhibition of vacuolar ATPase attenuates the TRAIL-induced activation of caspase-8 and modulates the trafficking of TRAIL receptosomes. The FEBS Journal, 280: 3436–3450. doi: 10.1111/febs.12347
- Issue online: 5 JUL 2013
- Version of Record online: 18 JUN 2013
- Accepted manuscript online: 16 MAY 2013 11:55PM EST
- Manuscript Accepted: 13 MAY 2013
- Manuscript Revised: 13 APR 2013
- Manuscript Received: 22 JAN 2013
- Czech Science Foundation. Grant Number: P301/10/1971 to LA and P301/11/1730
- Center of Molecular and Cellular Immunology. Grant Number: 1M6837805001
- Sixth Framework Program project ONCODEATH. Grant Number: AV0Z50520514
Fig. S1. V-ATPase inhibitors or chloroquine suppress to various extents TRAIL-induced apoptotic signalling in a number of human cancer cell lines.
Fig. S2. The inhibitory effect of bafilomycin A1 is fast and affects the proximal TRAIL-induced activation of caspase-8.
Fig. S3. Effect of concanamycin A on the distribution and trafficking of TRAIL-containing endosomes in DLD-1 cells.
Fig. S4. Downregulation of Rab7 GTPase has no effect on the activation of proximal TRAIL-induced apoptotic signalling.
Fig. S5. C18 sphingosine did not reverse the BafA1-mediated suppression of TRAIL-induced apoptotic signalling.
Fig. S6. Bafilomycin A1 treatment does not alter the cell surface expression of death receptors or the kinetics of their TRAIL-induced internalization in DLD-1 and Colo206F cells.
Fig. S7. Effect of suppressed endosomal acidification on the cleavage/processing of several apoptosis-related proteins and TRAIL-induced pro-survival signalling in DLD-1 and Colo206F cells.
Table S1. Sequences of short inhibitory RNAs in the shRNA-expressing lentiviral pLKO1-based vectors used for downregulation of cFLIP in DLD-1 cells.
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