Recent technological developments in proteomics shed new light on translational research on diabetic microangiopathy

Authors

  • Yuhang Ma,

    1. Department of Endocrinology and Metabolism, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
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  • Cheng Yang,

    1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
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  • Yimin Tao,

    1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
    2. CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
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  • Hu Zhou,

    Corresponding author
    1. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China
    2. CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China
    • Correspondence

      Y. Wang, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated First People's Hospital, 100 Haining Road, Shanghai 200080, China

      Fax: +86-21-63830185

      Tel: +86-21-63240090-5171

      E-mail: yyffwang@yahoo.com

      H. Zhou, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China

      Fax: +86-21-50806706

      Tel: +86-21-50806706

      E-mail: zhouhu@mail.shcnc.ac.cn

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  • Yufan Wang

    Corresponding author
    1. Department of Endocrinology and Metabolism, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai, China
    • Correspondence

      Y. Wang, Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated First People's Hospital, 100 Haining Road, Shanghai 200080, China

      Fax: +86-21-63830185

      Tel: +86-21-63240090-5171

      E-mail: yyffwang@yahoo.com

      H. Zhou, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China

      Fax: +86-21-50806706

      Tel: +86-21-50806706

      E-mail: zhouhu@mail.shcnc.ac.cn

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  • These authors contributed equally to this work.

Abstract

Diabetic microangiopathy has become a heavy social burden worldwide, but at present it is still difficult to predict and diagnose this ailment at an early stage. Various proteomics approaches have been applied to the pathophysiological study of diabetic microangiopathy. Conventional proteomics methods, including gel-based methods, exhibit limited sensitivity and robustness and have typically been used in high- or middle-abundance biomarker discovery. Clinical samples from patients with diabetic microangiopathy, such as biopsy samples, are minute in size. Therefore sample preparation, quantitative labelling and mass spectrometry technologies need to be optimized for low-abundance protein detection, multiple-sample processing and precision quantitation. In this review, we briefly introduce the recent technological developments in proteomics methods and summarize current proteomics-based, translational research on diabetic microangiopathy. Recent technological developments in proteomics tools may shed new light on the pathogenesis of diabetic microangiopathy and biomarkers and therapeutic targets related to this condition.

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