Consequences of proline-to-alanine substitutions for the stability and refolding of onconase
Article first published online: 12 JUL 2013
© 2013 FEBS
Volume 280, Issue 18, pages 4454–4462, September 2013
How to Cite
Hacke, M., Gruber, T., Schulenburg, C., Balbach, J. and Arnold, U. (2013), Consequences of proline-to-alanine substitutions for the stability and refolding of onconase. FEBS Journal, 280: 4454–4462. doi: 10.1111/febs.12406
- Issue published online: 2 SEP 2013
- Article first published online: 12 JUL 2013
- Accepted manuscript online: 24 JUN 2013 07:06AM EST
- Manuscript Accepted: 18 JUN 2013
- Manuscript Revised: 18 MAY 2013
- Manuscript Received: 1 MAR 2013
- European Regional Development Fund
- German Federal Ministry of Education and Research
Fig. S1. Thermally induced transition of ONC variants.
Fig. S2. Single-, double- and triple-exponential fits of the refolding kinetics.
Table S1. Mass spectrometry and activity data of ONC and its variants.
Table S2. Oligonucleotides for site-directed mutagenesis to generate the genes of the onconase (ONC) variants.
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