These authors contributed equally to this work.
Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns
Article first published online: 22 JUL 2013
© 2013 The Authors. FEBS Journal published by John Wiley & Sons Ltd on behalf of FEBS
This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Volume 280, Issue 18, pages 4474–4494, September 2013
How to Cite
Stiles, J. M., Pham, R., Rowntree, R. K., Amaya, C., Battiste, J., Boucheron, L. E., Mitchell, D. C. and Bryan, B. A. (2013), Morphological restriction of human coronary artery endothelial cells substantially impacts global gene expression patterns. FEBS Journal, 280: 4474–4494. doi: 10.1111/febs.12410
- Issue published online: 2 SEP 2013
- Article first published online: 22 JUL 2013
- Accepted manuscript online: 27 JUN 2013 03:10AM EST
- Manuscript Accepted: 24 JUN 2013
- Manuscript Revised: 6 MAY 2013
- Manuscript Received: 7 MAR 2013
- cell morphology;
- coronary artery;
- endothelial cell;
- image recognition;
Alterations in cell shape have been shown to modulate chromatin condensation and cell lineage specification; however, the mechanisms controlling these processes are largely unknown. Because endothelial cells experience cyclic mechanical changes from blood flow during normal physiological processes and disrupted mechanical changes as a result of abnormal blood flow, cell shape deformation and loss of polarization during coronary artery disease, we aimed to determine how morphological restriction affects global gene expression patterns. Human coronary artery endothelial cells (HCAECs) were cultured on spatially defined adhesive micropatterns, forcing them to conform to unique cellular morphologies differing in cellular polarization and angularity. We utilized pattern recognition algorithms and statistical analysis to validate the cytoskeletal pattern reproducibility and uniqueness of each micropattern, and performed microarray analysis on normal-shaped and micropatterned HCAECs to determine how constrained cellular morphology affects gene expression patterns. Analysis of the data revealed that forcing HCAECs to conform to geometrically-defined shapes significantly affects their global transcription patterns compared to nonrestricted shapes. Interestingly, gene expression patterns were altered in response to morphological restriction in general, although they were consistent regardless of the particular shape the cells conformed to. These data suggest that the ability of HCAECs to spread, although not necessarily their particular morphology, dictates their genomics patterns.