Structure of a bacterial glycoside hydrolase family 63 enzyme in complex with its glycosynthase product, and insights into the substrate specificity
Article first published online: 26 JUL 2013
© 2013 FEBS
Volume 280, Issue 18, pages 4560–4571, September 2013
How to Cite
Miyazaki, T., Ichikawa, M., Yokoi, G., Kitaoka, M., Mori, H., Kitano, Y., Nishikawa, A. and Tonozuka, T. (2013), Structure of a bacterial glycoside hydrolase family 63 enzyme in complex with its glycosynthase product, and insights into the substrate specificity. FEBS Journal, 280: 4560–4571. doi: 10.1111/febs.12424
- Issue published online: 2 SEP 2013
- Article first published online: 26 JUL 2013
- Accepted manuscript online: 4 JUL 2013 10:35AM EST
- Manuscript Accepted: 1 JUL 2013
- Manuscript Revised: 28 JUN 2013
- Manuscript Received: 8 MAY 2013
- Grant-in-Aid for Scientific Research. Grant Number: 23570132
- Research Fellowship from the Japan Society for the Promotion of Science
Fig. S1. Glycosynthase activity of YgjK E727A for a mixture of β-GlcF and monosaccharides.
Fig. S2. Schematic drawing of the YgjK glycosynthase reaction with a mixture of β-GlcF and Gal.
Fig. S3. Electron density maps for residues for which conformational changes are seen on ligand binding.
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