Cell cycle-dependent formation of Cdc45–Claspin complexes in human cells is compromized by UV-mediated DNA damage
Article first published online: 27 AUG 2013
© 2013 FEBS
Volume 280, Issue 19, pages 4888–4902, October 2013
How to Cite
Broderick, R., Rainey, M. D., Santocanale, C. and Nasheuer, H. P. (2013), Cell cycle-dependent formation of Cdc45–Claspin complexes in human cells is compromized by UV-mediated DNA damage. FEBS Journal, 280: 4888–4902. doi: 10.1111/febs.12465
- Issue published online: 17 SEP 2013
- Article first published online: 27 AUG 2013
- Accepted manuscript online: 2 AUG 2013 10:48AM EST
- Manuscript Accepted: 29 JUL 2013
- Manuscript Revised: 24 JUL 2013
- Manuscript Received: 6 SEP 2012
- Science Foundation Ireland (SFI). Grant Number: 07/RFP/GENF472
- Systems Biology Ireland
- Thomas Crawford Hayes Fund and Irish Research Council for Science, Engineering and Technology (IRCSET). Grant Number: 08/IN.1/B2064
Doc. S1. Drug validation.
Fig. S1. Localization of FLAG-Cdc45 and FLAG-Cdc45 mutants.
Fig. S2. Co-immunoprecipitation of FLAG-Cdc45 deletion mutants with replication proteins.
Fig. S3. Densitometry analysis of the Cdc45–Claspin interaction in the cell cycle.
Fig. S4. Densitometry analysis of the Cdc45–Claspin interaction after UVC treatment.
Fig. S5. FLAG immunoprecipitation in the presence of UCN-01.
Fig. S6. Validation of UCN-01 efficacy.
Fig. S7. Validation of caffeine efficacy.
Fig. S8. KU-55933 treatment does not rescue the Cdc45–Claspin interaction after UVC treatment.
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