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Fig. S1. Measurement of the diameter of the ATP nanosensor using dynamic light scattering.

Fig. S2. Calibration curves for the ATP sensor.

Fig. S3. Glycolytic oscillations measured as NADH fluorescence in the wild-type BY4743 strain (A), the hxk1Δ strain (B) and the hxk2Δ strain (C) following addition of glucose and KCN.

Fig. S4. Glycolytic oscillations measured as NADH fluorescence in the wild-type BY4743 strain (A), the hxk1Δ strain (B) and the hxk2Δ strain (C) following addition of mannose and KCN.

Fig. S5. Measurements of NADH fluorescence (A) and mitochondrial membrane potential (B) in the aac1Δ strain.

Fig. S6. Measurements of oxygen consumption of the BY4743 wild-type and in the corresponding strain in which the COX6 gene has been deleted.

Fig. S7. Effect of changing the hexokinase and phosphofructokinase activities on the frequency and amplitude of NADH oscillations in the Hald–Sørensen model of glycolysis.

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