Analysis of decapping scavenger cap complex using modified cap analogs reveals molecular determinants for efficient cap binding
Version of Record online: 23 OCT 2013
© 2013 FEBS
The FEBS Journal
Volume 280, Issue 24, pages 6508–6527, December 2013
How to Cite
Wypijewska del Nogal, A., Surleac, M. D., Kowalska, J., Lukaszewicz, M., Jemielity, J., Bisaillon, M., Darzynkiewicz, E., Milac, A. L. and Bojarska, E. (2013), Analysis of decapping scavenger cap complex using modified cap analogs reveals molecular determinants for efficient cap binding. The FEBS Journal, 280: 6508–6527. doi: 10.1111/febs.12553
- Issue online: 2 DEC 2013
- Version of Record online: 23 OCT 2013
- Accepted manuscript online: 1 OCT 2013 01:04AM EST
- Manuscript Accepted: 26 SEP 2013
- Manuscript Revised: 24 SEP 2013
- Manuscript Received: 6 AUG 2013
- Polish Ministry of Science and Higher Education. Grant Number: N 301 096 339
- National Science Centre. Grant Number: UMO-2012/05/E/ST5/03893
- National Center of Research and Development. Grant Number: 02/EuroNanoMed/2011
- University of Warsaw. Grant Number: 163500/BF
- Romanian Academy. Grant Number: 3
- European Social Fund. Grant Number: POSDRU/89/1.5/S/60746
- Romanian Ministry of Research and Education. Grant Number: ID3-0342-181/2011
Fig. S1. Calibration curve for molecular weight (MW) determination of C. elegans and A. suum DcpS proteins obtained by means of gel filtration.
Data S1. Materials and methods.
Table S1. Specificity and binding affinity of human DcpS towards cap analogs, determined in the same experimental conditions as for C. elegans DcpS: 50 mm Tris/HCl buffer containing 200 mm KCl, 0.5 mm EDTA and 1 mm dithiothreitol (pH 7.6), at 20 °C.
Table S2. Sampling parameters used in all docking experiments with autodock 4.2.
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