Kinetic analysis of cytochrome P450 reductase from Artemisia annua reveals accelerated rates of NADPH-dependent flavin reduction
Article first published online: 1 NOV 2013
© 2013 FEBS
Volume 280, Issue 24, pages 6627–6642, December 2013
How to Cite
Simtchouk, S., Eng, J. L., Meints, C. E., Makins, C. and Wolthers, K. R. (2013), Kinetic analysis of cytochrome P450 reductase from Artemisia annua reveals accelerated rates of NADPH-dependent flavin reduction. FEBS Journal, 280: 6627–6642. doi: 10.1111/febs.12567
- Issue published online: 2 DEC 2013
- Article first published online: 1 NOV 2013
- Accepted manuscript online: 12 OCT 2013 02:35AM EST
- Manuscript Accepted: 9 OCT 2013
- Manuscript Revised: 6 SEP 2013
- Manuscript Received: 8 FEB 2013
- Natural Sciences and Engineering Research Council of Canada
Fig. S1. The dependence of the rate of cytochrome c3+ reduction on the concentration of NADPH and cytochrome c3+.
Fig. S2. The dependence of the rate of cytochrome c3+ reduction on the concentration of exogenous FMN.
Fig. S3. Absorbance changes at 600 nm and 675 nm after the rapid mixing of 25 μm aaCPR and 250 μm NADPH.
Fig. S4. Stopped-flow absorbance traces at 454 nm after the mixing of 25.2 μm aaCPR with 250 μm NADPH at 6 °C and 25 °C.
Fig. S5. Photodiode array spectra after rapid mixing of 30 μm aaCPR against 22 mm NADH. The reaction was performed under anaerobic conditions in 50 mm Tris-HCl (pH 7.5) at 25 °C.
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