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Fig. S1. Nucleotide binding regulates complex formation.

Fig. S2. Representative fluorescence spectra of purified wild type and K422A MASL1 in the presence and absence of 6 m GdHCl using an excitation wavelength of 280 nm.

Fig. S3. SEC analysis of wild type MASL1 in the presence of 200 μm GTP or GMppCp.

Fig. S4. FACS analysis quantification.

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