Long non-coding RNA GHET1 promotes gastric carcinoma cell proliferation by increasing c-Myc mRNA stability

Authors

  • Feng Yang,

    Corresponding author
    1. Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    • Correspondence

      F. Yang, Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road No. 639, Shanghai 200011, China

      Fax: +86 21 23271697

      Tel: +86 21 23271697

      E-mail: yangfeng9hospital@163.com

      G. Fang, Department of General Surgery, Changhai Hospital, Second Military Medical University, Changhai Road No. 168, Shanghai 200433, China

      Fax: +86 21 81871114

      Tel: +86 21 81871114

      E-mail: gefangchanghai@sina.com

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  • Xuchao Xue,

    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
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  • Luming Zheng,

    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
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  • Jianwei Bi,

    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
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  • Yuhong Zhou,

    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
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  • Kangkang Zhi,

    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
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  • Yan Gu,

    1. Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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  • Guoen Fang

    Corresponding author
    1. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China
    • Correspondence

      F. Yang, Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Zhizaoju Road No. 639, Shanghai 200011, China

      Fax: +86 21 23271697

      Tel: +86 21 23271697

      E-mail: yangfeng9hospital@163.com

      G. Fang, Department of General Surgery, Changhai Hospital, Second Military Medical University, Changhai Road No. 168, Shanghai 200433, China

      Fax: +86 21 81871114

      Tel: +86 21 81871114

      E-mail: gefangchanghai@sina.com

    Search for more papers by this author

Abstract

Long non-coding RNAs (lncRNAs), a recently characterized class of non-coding RNAs, have been shown to have important regulatory roles and are de-regulated in a variety of tumors. However, the contributions of lncRNAs to gastric carcinoma and their functional mechanisms remain largely unknown. In this study, we found that lncRNA gastric carcinoma high expressed transcript 1 (lncRNA-GHET1) was up-regulated in gastric carcinoma. The over-expression of this lncRNA correlates with tumor size, tumor invasion and poor survival. Gain-of-function and loss-of-function analyses demonstrated that GHET1 over-expression promotes the proliferation of gastric carcinoma cells in vitro and in vivo. Knockdown of GHET1 inhibits the proliferation of gastric carcinoma cells. RNA pull-down and immunoprecipitation assays confirmed that GHET1 physically associates with insulin-like growth factor 2 mRNA binding protein 1 (IGF2BP1) and enhances the physical interaction between c-Myc mRNA and IGF2BP1, consequently increasing the stability of c-Myc mRNA and expression. The expression of GHET1 and c-Myc is strongly correlated in gastric carcinoma tissues. Depletion of c-Myc abolishes the effects of GHET1 on proliferation of gastric carcinoma cells. Taken together, these findings indicate that GHET1 plays a pivotal role in gastric carcinoma cell proliferation via increasing c-Myc mRNA stability and expression, which suggests potential use of GHET1 for the prognosis and treatment of gastric carcinoma.

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