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Fig. S1. UV-visible absorption spectra of WT and 3Glu recorded at increasing temperatures.

Fig. S2. Plots of the absorption changes at 560, 460 and 370 nm as a function of temperature for WT and 3Glu.

Fig. S3. Infrared absorption spectra of amide I and amide II regions of WT and 3Glu.

Fig. S4. Representative differential scanning calorimetry traces for WT and 3Glu.

Doc. S1. Supporting materials and methods.

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