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Fig. S1. SDS/PAGE profiles showing equal loading for in vitro and in vivo coimmunoprecipitation reactions of PPE68-His6 and GST–PE35.

Fig. S2. Subcellular localization and surface accessibility of PE35 and PPE68.

Fig. S3. Growth of recombinant Mycobacterium smegmatis expressing PE35 and PPE68.

Fig. S4. Real-time RT-PCR quantification of PE35, PPE68 and PE35–PPE68 transcripts as a function of growth.

Fig. S5. Effect of MAPK inhibition on PE35-stimulated, PPE68-stimulated and PE35/PPE68-stimulated IL-10 and MCP-1 expression.

Fig. S6. Effect of TLR2 blockade on PE35-stimulated, PPE68-stimulated and PE35/PPE68-stimulated IL-10 and MCP-1 expression.

Fig. S7. MCP-1 levels in the culture supernatants of resting THP-1 macrophages infected with Mycobacterium smegmatis strains expressing pMV261, PE5 (Rv0285), and PE15 (Rv1386), 24 h postinfection.

Table S1. Oligonucleotides used in this study.

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