An immunomodulatory role for the Mycobacterium tuberculosis region of difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873)
Article first published online: 12 FEB 2014
© 2014 FEBS
Volume 281, Issue 6, pages 1556–1570, March 2014
How to Cite
Tiwari, B., Soory, A. and Raghunand, T. R. (2014), An immunomodulatory role for the Mycobacterium tuberculosis region of difference 1 locus proteins PE35 (Rv3872) and PPE68 (Rv3873). FEBS Journal, 281: 1556–1570. doi: 10.1111/febs.12723
- Issue published online: 18 MAR 2014
- Article first published online: 12 FEB 2014
- Accepted manuscript online: 27 JAN 2014 09:06PM EST
- Manuscript Accepted: 21 JAN 2014
- Manuscript Revised: 3 DEC 2013
- Manuscript Received: 2 OCT 2013
- Department of Science and Technology (DST), Government of India. Grant Number: SR/SO/HS-115/2009
- Council of Scientific and Industrial Research, Government of India
Fig. S1. SDS/PAGE profiles showing equal loading for in vitro and in vivo coimmunoprecipitation reactions of PPE68-His6 and GST–PE35.
Fig. S2. Subcellular localization and surface accessibility of PE35 and PPE68.
Fig. S3. Growth of recombinant Mycobacterium smegmatis expressing PE35 and PPE68.
Fig. S4. Real-time RT-PCR quantification of PE35, PPE68 and PE35–PPE68 transcripts as a function of growth.
Fig. S5. Effect of MAPK inhibition on PE35-stimulated, PPE68-stimulated and PE35/PPE68-stimulated IL-10 and MCP-1 expression.
Fig. S6. Effect of TLR2 blockade on PE35-stimulated, PPE68-stimulated and PE35/PPE68-stimulated IL-10 and MCP-1 expression.
Fig. S7. MCP-1 levels in the culture supernatants of resting THP-1 macrophages infected with Mycobacterium smegmatis strains expressing pMV261, PE5 (Rv0285), and PE15 (Rv1386), 24 h postinfection.
Table S1. Oligonucleotides used in this study.
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