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Fig. S1. The Cα rmsd of the ABCB1 TMDs after labelling introduced cysteine residues with MTSL.

Fig. S2. Accessibility of MTSL-labelled residues to quenching agents.

Fig. S3. EPR accessibility measurements.

Fig. S4. Orientation of the MTSL moiety in ABCB1 mutant isoforms.

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