An anionic synthetic sugar containing 6-SO3-NAcGlc mimics the sulfated cruzipain epitope that plays a central role in immune recognition (pages 3665–3679)
Alicia S. Couto, Luciana L. Soprano, Malena Landoni, Marilyne Pourcelot, Diana M. Acosta, Laurent Bultel, Juliana Parente, Maximiliano R. Ferrero, Maximilien Barbier, Christophe Dussouy, Mónica I. Esteva, José Kovensky and Vilma G. Duschak
Article first published online: 4 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08728.x
A systematic study of the chemical characteristics needed for antibody binding to the cruzipain sulfated epitope was performed by immunoassays. Different molecules were synthesized, coupled to BSA/aprotinin, and confronted with rabbit/mice sera specific for Cz/C-T and purified IgGs from immune and human Chagas disease sera, demonstrating that synthetic GlcNAc6S mimics the N-glycan-linked-sulfated epitope displayed in the C-T domain of natural cruzipain.