You have free access to this content

The FEBS Journal

Cover image for Vol. 279 Issue 20

October 2012

Volume 279, Issue 20

Pages 3765–3996

  1. Review Article

    1. Top of page
    2. Review Article
    3. Original Articles
    4. Author index
    1. You have free access to this content
      Beyond genotype to phenotype: why the phenotype of an individual cannot always be predicted from their genome sequence and the environment that they experience (pages 3765–3775)

      Alejandro Burga and Ben Lehner

      Version of Record online: 27 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08810.x

      Thumbnail image of graphical abstract

      What determines the phenotype of an individual? In this review we highlight recent mechanistic studies dissecting why the phenotypes of individuals cannot always be reliably predicted from their genotypes. This includes understanding the consequences of stochastic noise, parental non-inherited mutations, and trans-generational effects of the environment

  2. Original Articles

    1. Top of page
    2. Review Article
    3. Original Articles
    4. Author index
    1. You have free access to this content
      Comparative mode of action of novel hybrid peptide CS-1a and its rearranged amphipathic analogue CS-2a (pages 3776–3790)

      Seema Joshi, Gopal S. Bisht, Diwan S. Rawat, Souvik Maiti and Santosh Pasha

      Version of Record online: 7 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08738.x

      Thumbnail image of graphical abstract

      Here we report synthesis of two novel, cell selective peptides with potent activity against clinically relevant pathogens Acenatobacter sp. and methicillin resistant S. aureus. Biophysical and microscopic evidence showed that peptides with discontinuous hydrophobic and charged sectors showed diminished integration into bacterial/mammalian cells, which may be utilized as a strategy for designing better peptides with therapeutic potential

      Corrected by:

      Corrigendum/Erratum: Corrigendum/Erratum

      Vol. 279, Issue 23, 4421, Version of Record online: 22 OCT 2012

    2. You have free access to this content
      Identification of potent small-molecule inhibitors of STAT3 with anti-inflammatory properties in RAW 264.7 macrophages (pages 3791–3799)

      Hemachander Capiralla, Valérie Vingtdeux, Jeganathan Venkatesh, Ute Dreses-Werringloer, Haitian Zhao, Peter Davies and Philippe Marambaud

      Version of Record online: 4 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08739.x

      Thumbnail image of graphical abstract

      Signal transducer and activator of transcription 3 (STAT3) is a key mediator of the inflammatory response in several immune cell types. Here, we show that the small-molecule analogs of resveratrol, RSVA314 and RSVA405, are potent inhibitors of STAT3 and are associated with anti-inflammatory effects in macrophages. The small-molecules act by promoting the dephosphorylation of STAT3 by protein tyrosine phosphatases

    3. You have free access to this content
      MicroRNA-133b inhibits the growth of non-small-cell lung cancer by targeting the epidermal growth factor receptor (pages 3800–3812)

      Lingxiang Liu, Xiaoyan Shao, Wen Gao, Zhi Zhang, Ping Liu, Rongsheng Wang, Puwen Huang, Yongmei Yin and Yongqian Shu

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08741.x

      Thumbnail image of graphical abstract

      MiR–133b was found to be associated with tumor stage, the extent of regional lymph node involvement stage, visceral pleura or vessel invasion and EGFR mRNA expression in Chinese patients with NSCLC. Moreover, miR–133b can inhibit cell growth of NSCLC through targeting EGFR and regulating its downstream signaling pathway, especially in EGFR–addicted NSCLC cells

    4. You have free access to this content
      Identification and characterization of a cis,trans-mixed heptaprenyl diphosphate synthase from Arabidopsis thaliana (pages 3813–3827)

      Kota Kera, Seiji Takahashi, Tsuyoshi Sutoh, Tanetoshi Koyama and Toru Nakayama

      Version of Record online: 7 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08742.x

      Thumbnail image of graphical abstract

      From Arabidopsis thaliana, AtHEPS was identified as a novel plant cis, trans-mixed heptaprenyl diphosphate synthase, responsible for the formation of C35 betulaprenol (di-trans, tetra-cis-polyprenol). Subcellular localization of AtHEPS on the ER was shown by using GFP-fused proteins. A cold-stress-inducible expression of AtHEPS suggested that AtHEPS and its product might function in response to abiotic stresses on the ER

    5. You have free access to this content
      Functional and structural studies of the disulfide isomerase DsbC from the plant pathogen Xylella fastidiosa reveals a redox-dependent oligomeric modulation in vitro (pages 3828–3843)

      Clelton A. Santos, Marcelo A. S. Toledo, Daniela B. B. Trivella, Lilian L. Beloti, Dilaine R. S. Schneider, Antonio M. Saraiva, Aline Crucello, Adriano R. Azzoni, Alessandra A. Souza, Ricardo Aparicio and Anete P. Souza

      Version of Record online: 7 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08743.x

      Thumbnail image of graphical abstract

      DsbC is part of the disulfide bond reduction/isomerization pathway in the bacterial periplasm. Using biochemical and biophysical techniques, we characterized the XfDsbC from Xylella fastidiosa. Based on SAXS data and DLS analysis, we reported a redox-dependent oligomeric modulation of XfDsbC which may be involved in substrate recognition and specificity, a role that has not been previously described for DsbC homologs

    6. You have free access to this content
      The proline-rich tetramerization peptides in equine serum butyrylcholinesterase (pages 3844–3858)

      Kevser Biberoglu, Lawrence M. Schopfer, Ozden Tacal and Oksana Lockridge

      Version of Record online: 7 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08744.x

      Thumbnail image of graphical abstract

      Boiling soluble, tetrameric, plasma butyrylcholinesterase from horse released a set of polyproline peptides ranging in mass from 1173 to 2098 Da. These peptides contained a minimum of 11 consecutive proline residues and as many as 21. Many of these peptides also included non-proline amino acids at their N-terminus. These peptides promote tetramer organization

    7. You have free access to this content
      Hepatitis B virus X protein and c-Myc cooperate in the upregulation of ribosome biogenesis and in cellular transformation (pages 3859–3871)

      Surendra Kumar Shukla and Vijay Kumar

      Version of Record online: 7 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08745.x

      Thumbnail image of graphical abstract

      The present study on oncogenic cooperation between HBx and c-Myc genes showed enhanced ribosomal synthesis combined with increased cell proliferation and transformation of immortalized human hepatocytes. Elevated hepatic levels of rRNAs were also observed in a transgenic HBx-Myc microenvironment. Thus, HBx and c-Myc seem to work cooperatively to support ribosome biogenesis and cellular transformation

    8. You have free access to this content
      A novel trafficking pathway in Plasmodium falciparum for the organellar localization of glutathione peroxidase-like thioredoxin peroxidase (pages 3872–3888)

      Rahul Chaudhari, Aishwarya Narayan and Swati Patankar

      Version of Record online: 13 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08746.x

      Thumbnail image of graphical abstract

      P. falciparum glutathione peroxidase-like thioredoxin peroxidase (PfTPxGl), we show, is localized to the apicoplast, the mitochondrion and the cytosol by a non-canonical pathway involving the ER and Golgi. The localization of PfTPxGl was heterogeneous in the population. In the context of evolution, the primitive ER-Golgi route may have been retained as opposed to optimization for individual organellar import pathways

    9. You have free access to this content
      Two thymidine kinases and one multisubstrate deoxyribonucleoside kinase salvage DNA precursors in Arabidopsis thaliana (pages 3889–3897)

      Anders R. Clausen, Lenart Girandon, Ashfaq Ali, Wolfgang Knecht, Elzbieta Rozpedowska, Michael P. B. Sandrini, Erik Andreasson, Birgitte Munch-Petersen and Jure Piškur

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08747.x

      Thumbnail image of graphical abstract

      Deoxyribonucleoside kinases (dNKs) phosphorylate deoxyribonucleosides to deoxyribonucleoside mono-phosphates. We report that the plant Arabidopsis thaliana genome encodes three dNKs. The corresponding genes were overexpressed and two dNKs behaved as thymidine kinases (TK1s), and one as a multi-substrate dNK. If any of the two TK1s was knocked out, the plant was viable, while a double knock-out died during early development

    10. You have free access to this content
      Knockdown of lactate dehydrogenase A suppresses tumor growth and metastasis of human hepatocellular carcinoma (pages 3898–3910)

      Shi L. Sheng, Jian J. Liu, Yun H. Dai, Xiao G. Sun, Xiao P. Xiong and Gang Huang

      Version of Record online: 13 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08748.x

      Thumbnail image of graphical abstract

      Our studies reveal that previously unidentified effects of LHDA may mediate tumor growth and metastasic effects in HCC. LDHA inhibition results in increased apoptosis via ROS production in HCCLM3 cells. LDHA knockdown results in significant reduction in metastatic potential in a xenograft mouse model. These studies have important implications for understanding the mechanisms by which LDHA promotes tumor growth and metastasis

    11. You have free access to this content
      In vivo and in vitro studies on the carotenoid cleavage oxygenases from Sphingopyxis alaskensis  RB2256 and Plesiocystis pacifica SIR-1 revealed their substrate specificities and non-retinal-forming cleavage activities (pages 3911–3924)

      Jana Hoffmann, Judit Bóna-Lovász, Holger Beuttler and Josef Altenbuchner

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08751.x

      Thumbnail image of graphical abstract

      The substrates of carotenoid cleavage oxygenase (PpCCO) from Plesiocystis pacifica SIR-1 have something in common: hydroxy or keto groups. When these functional groups are present, the enzyme cuts acyclic and bicyclic C40 carotenoids and also C50 carotenoids. SaCCO from Sphingopyxis alaskensis RB2256 cleaves acyclic and monocyclic carotenoids and apocarotenoids. The enzyme was biochemically characterised by in vitro incubations with apo-8′-carotenal

    12. You have free access to this content
      The P1/P2 proteins of the human ribosomal stalk are required for ribosome binding and depurination by ricin in human cells (pages 3925–3936)

      Kerrie L. May, Xiao-Ping Li, Francisco Martínez-Azorín, Juan P. G. Ballesta, Przemysław Grela, Marek Tchórzewski and Nilgun E. Tumer

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08752.x

      Thumbnail image of graphical abstract

      We examine if ribosomal stalk proteins facilitate the activity of ricin A-chain (RTA) in human cells. Knockdown of P1/P2 in human cells reduces depurination by RTA. RTA interacts directly with recombinant human P1/P2 and exhibits reduced binding and depurination of P1/P2-depleted ribosomes. This study demonstrates the importance of stalk proteins for the interaction of RTA with ribosomes in human cells

    13. You have free access to this content
      X-ray structure of a protease-resistant mutant form of human galectin-8 with two carbohydrate recognition domains (pages 3937–3951)

      Hiromi Yoshida, Satoshi Yamashita, Misa Teraoka, Aiko Itoh, Shin-ichi Nakakita, Nozomu Nishi and Shigehiro Kamitori

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08753.x

      Thumbnail image of graphical abstract

      Galectin-8 is a tandem-repeat-type β-galactoside-specific animal lectin having N-terminal and C-terminal carbohydrate recognition domains (CRD). No X-ray structure of a galectin containing both CRDs has been reported. The X-ray structure of a mutant form of human galectin-8 having both CRDs was determined, revealing a difference in specificity between the CRDs, and providing new insights into the molecular association of galectin-8

    14. You have free access to this content
      Structural determination of the phosphorylation domain of the ryanodine receptor (pages 3952–3964)

      Parveen Sharma, Noboru Ishiyama, Usha Nair, Wenping Li, Aiping Dong, Tetsuaki Miyake, Aaron Wilson, Tim Ryan, David H. MacLennan, Thomas Kislinger, Mitsuhiko Ikura, Sirano Dhe-Paganon and Anthony O. Gramolini

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08755.x

      Thumbnail image of graphical abstract

      The ryanodine receptor (RyR1) is an essential protein for calcium cycling in both skeletal and cardiac muscle. We present a 2.2Å resolution crystal structure of the phosphorylation domain of RyR1 revealing a two-fold symmetric, four-helix bundle structure stabilized by two β-sheets. Modelling localized this structure into the dynamic clamp region of RyR1, a region absent from the inositol 1,4,5-trisphosphate receptor

    15. You have free access to this content
      Probing murine methyltransfease Dnmt3a interactions with benzo[a]pyrene-modified DNA by fluorescence methods (pages 3965–3980)

      Antonio S. Minero, Olga V. Lukashevich, Natalia A. Cherepanova, Alexander Kolbanovskiy, Nicholas E. Geacintov and Elizaveta S. Gromova

      Version of Record online: 11 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08756.x

      Thumbnail image of graphical abstract

      The impact of the stereoisomeric benzo[a]pyrene diol epoxide (B[a]PDE)-derived guanine or adenine adducts on the methylation and fluorescence of the covalently bound B[a]PDE residues upon complex formation with the Dnmt3a catalytic domain were studied. The results are consistent with a flipping of the target cytosine and a motion of the Dnmt3a catalytic loop that results in a change in B[a]PDE fluorescence (multipointed stars)

    16. You have free access to this content
      A non-canonical caleosin from Arabidopsis efficiently epoxidizes physiological unsaturated fatty acids with complete stereoselectivity (pages 3981–3995)

      Elizabeth Blée, Martine Flenet, Benoît Boachon and Marie-Laure Fauconnier

      Version of Record online: 14 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08757.x

      Thumbnail image of graphical abstract

      No genes encoding plant oxygenases responsible for the formation of fatty acid epoxides are presently known, aside from vernolic acid which is present in seeds. We report that PXG4 encodes a non-seed specific caleosin able to efficiently catalyze the epoxidation of the double bonds of unsaturated fatty acids with a very high stereoselectivity, giving access to the stereoisomers found in planta

  3. Author index

    1. Top of page
    2. Review Article
    3. Original Articles
    4. Author index
    1. You have free access to this content
      Author index (page 3996)

      Version of Record online: 2 OCT 2012 | DOI: 10.1111/j.1742-4658.2012.08340.x

SEARCH

SEARCH BY CITATION