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The FEBS Journal

Cover image for Vol. 279 Issue 21

November 2012

Volume 279, Issue 21

Pages 3997–4110

  1. Original Articles

    1. Top of page
    2. Original Articles
    3. Corrigendum
    4. Author index
    1. You have free access to this content
      Subtilisin-like proprotein convertase paired basic amino acid-cleaving enzyme 4 is required for chondrogenic differentiation in ATDC5 cells (pages 3997–4009)

      Keizo Yuasa, Go Futamatsu, Tsuyoshi Kawano, Masaki Muroshita, Yoko Kageyama, Hiromi Taichi, Hiroshi Ishikawa, Masami Nagahama, Yoshiko Matsuda and Akihiko Tsuji

      Version of Record online: 17 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08758.x

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      Bone morphogenetic proteins (BMPs) are implicated in the regulation of multiple stages of endochondral bone development. The SPC family proteolytically activates various signaling molecules including BMPs. PACE4 mRNA levels increased markedly during chondrogenic differentiation in ATDC5 cells, whereas the furin expression remained unchanged. Knockdown of PACE4 expression reduced chondrogenic differentiation. These results indicate that PACE4 is required for chondrogenic differentiation in ATDC5 cells.

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      Lys66 residue as a determinant of high mismatch extension and misinsertion rates of HIV-1 reverse transcriptase (pages 4010–4024)

      Chisanga Lwatula, Scott J. Garforth and Vinayaka R. Prasad

      Version of Record online: 27 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08807.x

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      A major factor contributing to the high mutation rate of HIV-1 RT is its high propensity for misincorporation. Amino acid subsitutions at position Lys66 show 4 to 6-fold and 3 to 23-fold reduction in efficiencies of mismatch extension and misinsertion respectively. Our results suggest that Lys66 is a determinant of the overall miscorporation fidelity of HIV-1 RT.

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      Subtype specificity interaction of bactridines with mammalian, insect and bacterial sodium channels under voltage clamp conditions (pages 4025–4038)

      Steve Peigneur, Carlos Sevcik, Jan Tytgat, Cecilia Castillo and Gina D'Suze

      Version of Record online: 1 OCT 2012 | DOI: 10.1111/j.1742-4658.2012.08808.x

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      Bactridines are scorpion toxins combining a dual functionality as antibacterial toxins and as selective voltage-gated sodium channel modulators. Bactridines possess different selectivities for neuronal and muscular NaV channels, with subtle differences in their effects on different channel isoforms. Bactridines emerge as potential research tools to understand sodium channel isoforms structure-function relationships, and also as pharmacologically interesting peptides.

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      Single point mutation in Vibrio cholerae cytolysin compromises the membrane pore-formation mechanism of the toxin (pages 4039–4051)

      Karan Paul and Kausik Chattopadhyay

      Version of Record online: 27 SEP 2012 | DOI: 10.1111/j.1742-4658.2012.08809.x

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      Vibrio cholerae cytolysin (VCC) belongs to the family of -barrel pore-forming protein toxins ( -PFTs). Single point mutation of Ala425Val positioned within a potential membrane-interacting loop in the VCC structure critically compromises its cholesterol-dependent membrane interaction mechanism, and also abrogates the process of functional membrane pore formation by the toxin.

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      Comparative structural and functional analysis of two octaheme nitrite reductases from closely related Thioalkalivibrio species (pages 4052–4061)

      Tamara Tikhonova, Alexey Tikhonov, Anton Trofimov, Konstantin Polyakov, Konstantin Boyko, Eugene Cherkashin, Tatiana Rakitina, Dmitry Sorokin and Vladimir Popov

      Version of Record online: 12 OCT 2012 | DOI: 10.1111/j.1742-4658.2012.08811.x

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      A comparative structural-functional analysis of two homologous octaheme nitrite reductases was performed. Both enzymes are characterized by specific structural features, which are responsible for the higher nitrite reductase activity, the greater preference for nitrite than sulfite as a substrate, and the wider __ range of the catalytic activity of octaheme nitrite reductases compared to pentaheme homologues.

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      Tumour suppressor p16INK4a – anoikis-favouring decrease in N/O-glycan/cell surface sialylation by down-regulation of enzymes in sialic acid biosynthesis in tandem in a pancreatic carcinoma model (pages 4062–4080)

      Maho Amano, Hanna Eriksson, Joachim C. Manning, Katharina M. Detjen, Sabine André, Shin-Ichiro Nishimura, Janne Lehtiö and Hans-Joachim Gabius

      Version of Record online: 12 OCT 2012 | DOI: 10.1111/febs.12001

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      The tumor suppressor p16INK4a, a well-known control element of the cell cycle, also increases susceptibility to anoikis. Toward this end, an effector lectin (galectin-1) is upregulated and glycosylation reprogrammed.  2,6-Sialylation, which precludes galectin-1 reactivity, is decreased by downregulation sialic acid biosynthesis at the level of expression of two enzymes of this pathway in tandem.

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      Role of DNA methylation in growth and differentiation in Physcomitrella patens and characterization of cytosine DNA methyltransferases (pages 4081–4094)

      Garima Malik, Meenakshi Dangwal, Sanjay Kapoor and Meenu Kapoor

      Version of Record online: 1 OCT 2012 | DOI: 10.1111/febs.12002

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      The role of DNA methylation on various aspects of development in Physcomitrella patens has been analyzed. A genome-wide search reveals seven cytosine DNA methyltransferases, five of which are well conserved, while two are specific to P. patens. All seven genes are differentially and ubiquitously expressed. Sub-cellular localization patterns of GFP-fused proteins have been analyzed. This work provides knowledge of methyltransferases in P. patens, one of the simplest land plants.

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      A rising tide of blue-absorbing biliprotein photoreceptors – characterization of seven such bilin-binding GAF domains in Nostoc sp. PCC7120 (pages 4095–4108)

      Qiong Ma, Huan-Huan Hua, Yu Chen, Bin-Bin Liu, Anna Laura Krämer, Hugo Scheer, Kai-Hong Zhao and Ming Zhou

      Version of Record online: 1 OCT 2012 | DOI: 10.1111/febs.12003

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      Seven DXCF GAFs of cyanobacteriochromes are identified in Nostoc sp. PCC 7120. The GAFs, which are similar to those from Nostoc punctiforme, covalently bind bilin(s) and show photoreversibly photochromic responses with a common absorption around 420 nm in the 15Z states, and a variety of red-shifted absorption in the 490 – 600 nm range in the 15E states.

  2. Corrigendum

    1. Top of page
    2. Original Articles
    3. Corrigendum
    4. Author index
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      Corrigendum (page 4109)

      Version of Record online: 8 OCT 2012 | DOI: 10.1111/febs.12011

      This article corrects:

      Peptides that inhibit HIV-1 integrase by blocking its protein–protein interactions

      Vol. 279, Issue 16, 2795–2809, Version of Record online: 23 JUL 2012

  3. Author index

    1. Top of page
    2. Original Articles
    3. Corrigendum
    4. Author index
    1. You have free access to this content
      Author index (page 4110)

      Version of Record online: 12 OCT 2012 | DOI: 10.1111/j.1742-4658.2012.08341.x

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