pH modulates the binding of early growth response protein 1 transcription factor to DNA (pages 3669–3684)
David C. Mikles, Vikas Bhat, Brett J. Schuchardt, Brian J. Deegan, Kenneth L. Seldeen, Caleb B. McDonald and Amjad Farooq
Article first published online: 18 JUN 2013 | DOI: 10.1111/febs.12360
We show that the binding of EGR1 transcription factor to DNA is tightly regulated by solution pH by virtue of the ability of His382 to undergo protonation-deprotonation equilibrium. Remarkably, His382 is predominantly conserved across other members of EGR1 family, implying that histidine protonation-deprotonation may serve as a molecular switch for modulating protein-DNA interactions central to this family of transcription factors.