Global characterization of signalling networks associated with tamoxifen resistance in breast cancer (pages 5237–5257)
Brigid C. Browne, Falko Hochgräfe, Jianmin Wu, Ewan K. A. Millar, Jane Barraclough, Andrew Stone, Rachael A. McCloy, Christine S. Lee, Caroline Roberts, Naveid A. Ali, Alice Boulghourjian, Fabian Schmich, Rune Linding, Lynn Farrow, Julia M. W. Gee, Robert I. Nicholson, Sandra A. O'Toole, Robert L. Sutherland, Elizabeth A. Musgrove, Alison J. Butt and Roger J. Daly
Article first published online: 19 AUG 2013 | DOI: 10.1111/febs.12441
In this study, tamoxifen-resistant MCF7 breast cancer cells (TamR) were characterized using proteomics and phosphoproteomics. Phosphorylation of Yes kinase and expression of actin-binding protein MARCKS were significantly elevated in TamR cells, and each were found to contribute to TamR cell motility. In primary breast cancers, MARCKS was independently predictive of poor survival in the whole cohort and in ER-positive patients.