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The FEBS Journal

Cover image for Vol. 280 Issue 5

March 2013

Volume 280, Issue 5

Pages i–iii, 1169–1380

  1. Front Cover

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
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      Front Cover (page i)

      Version of Record online: 26 FEB 2013 | DOI: 10.1111/febs.8768

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      Resveratrol and nitric oxide production in macrophages by V. J. Koller et al. (pp. 1214–1225).

  2. Editorial Information

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
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      Editorial Information (pages ii–iii)

      Version of Record online: 26 FEB 2013 | DOI: 10.1111/febs.8768_1

  3. Review Article

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
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      Structural and functional aspects of PR-10 proteins (pages 1169–1199)

      Humberto Fernandes, Karolina Michalska, Michal Sikorski and Mariusz Jaskolski

      Version of Record online: 6 FEB 2013 | DOI: 10.1111/febs.12114

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      Despite their abundance and numerous studies, the role of plant pathogenesis related class 10 (PR–10) proteins is obscure. They have a distinct fold, with a huge hydrophobic internal cavity that can accommodate a great variety of biologically important molecules, including different phytohormones. The emerging structural picture strongly suggests that PR–10 proteins could function as versatile binders of key signaling molecules

  4. Original Articles

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
    1. You have free access to this content
      Tetraspanin protein CD9 interacts with metalloprotease CD10 and enhances its release via exosomes (pages 1200–1213)

      Dmitriy Mazurov, Lubov Barbashova and Alexander Filatov

      Version of Record online: 6 FEB 2013 | DOI: 10.1111/febs.12110

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      Tetraspanins interact with transmembrane proteins called molecular partners. We describe a new partner of tetraspanin CD9, metalloprotease CD10. Using mutagenic analysis we determine that large extracellular loop and cytoplasmic C-terminus of CD9 are involved in the interaction with CD10. Association of CD10 with CD9 enhances the release of CD10 with exosomes. This may facilitate pre–B cell migration in bone marrow

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      Modulation of bacterial ghosts – induced nitric oxide production in macrophages by bacterial ghost-delivered resveratrol (pages 1214–1225)

      Verena J. Koller, Verena M. Dirsch, Hortenzia Beres, Oliver Donath, Gottfried Reznicek, Werner Lubitz and Pavol Kudela

      Version of Record online: 31 JAN 2013 | DOI: 10.1111/febs.12112

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      The present study demonstrates that NO formation induced after internalization of BGs by macrophages can be effectively modulated by RV loaded inside BGs and delivered directly to the cytosol of target cells. Low doses of RV presented within the BG's envelope significantly decreased generation of NO without having a cytotoxic impact on the viability of phagocytic cells

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      Spore germination of Trichoderma atroviride is inhibited by its LysM protein TAL6 (pages 1226–1236)

      Verena Seidl-Seiboth, Simone Zach, Alexa Frischmann, Oliver Spadiut, Christian Dietzsch, Christoph Herwig, Claudia Ruth, Agnes Rodler, Alois Jungbauer and Christian P. Kubicek

      Version of Record online: 31 JAN 2013 | DOI: 10.1111/febs.12113

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      LysM motifs have general chitin-binding properties and fungal LysM proteins are involved in fungal-plant interactions. We investigated the LysM protein TAL6 from Trichoderma atroviride. The results showed that TAL6 inhibits spore germination of Trichoderma species, but interestingly not of other fungi. This expands the functions of fungal LysM proteins to roles in the self-regulation of fungal growth and development

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      Translational enhancing activity in 5′ UTR of peste des petits ruminants virus fusion gene (pages 1237–1248)

      Songkhla Chulakasian, Tien-Jye Chang, Ching-Hsiu Tsai, Min-Liang Wong and Wei-Li Hsu

      Version of Record online: 30 JAN 2013 | DOI: 10.1111/febs.12115

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      We report a translational enhancer in PPRV-F 5′ UTR, independent of cell- and gene-specificity. Northern blotting analysis suggested elevated gene expression was accompanied by an increased mRNA level and enhanced mRNA stability. Moreover, deletion analysis identified the complementary sequence to 18S rRNA and distal nucleotides are necessary for the enhancing activity driven by PPRV- F 5′ UTR

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      Anterior gradient 2 and 3 – two prototype androgen-responsive genes transcriptionally upregulated by androgens and by oestrogens in prostate cancer cells (pages 1249–1266)

      Huajie Bu, Michal R. Schweiger, Thomas Manke, Andrea Wunderlich, Bernd Timmermann, Martin Kerick, Lorenza Pasqualini, Erald Shehu, Christian Fuchsberger, Andrew C. B. Cato and Helmut Klocker

      Version of Record online: 13 FEB 2013 | DOI: 10.1111/febs.12118

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      Prostate cancer associated anterior gradient 2 (AGR2) and its homolog AGR3 were characterized as androgen responsive genes. Androgen regulation involves androgen receptor (AR) binding sites in the distal promoter of AGR2 and a binding site in the first intron of AGR3. Besides androgen also oestrogen induces ARG2 and 3 transcription through promiscuous activation of a high-level of wild-type AR

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      Purification, kinetics, inhibitors and CD for recombinant β-amyrin synthase from Euphorbia tirucalli L and functional analysis of the DCTA motif, which is highly conserved among oxidosqualene cyclases (pages 1267–1280)

      Ryousuke Ito, Yukari Masukawa and Tsutomu Hoshino

      Version of Record online: 13 FEB 2013 | DOI: 10.1111/febs.12119

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      First detailed characterization of plant β-amyrin synthase is reported including Km, kcat and IC50s of inhibitors and identification of active site residues responsible for the polycyclization initiation. Furthermore, the CD spectrum is reported as the first case of oxidosqualene cyclases

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      Ubiquitin-like protein MNSFβ covalently binds to Bcl–G and enhances lipopolysaccharide/interferon γ-induced apoptosis in macrophages (pages 1281–1293)

      Jun Watanabe, Mai Nakagawa, Natsuko Watanabe and Morihiko Nakamura

      Version of Record online: 11 FEB 2013 | DOI: 10.1111/febs.12120

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      Co-overexpression of ubiquitin-like protein MNSFβ and pro-apoptotic protein Bcl-G greatly enhanced LPS/IFNγ-induced apoptosis in Raw264.7 cells, accompanied with an increased expression of p53 and a decreased Cox-2 activity. MNSFβ down-regulates ERK/AP-1 signaling cascade leading to Cox-2 activation. Our data indicate an apoptosis enhancing effect of MNSFβ•Bcl-G is due in part to the down-regulation of Cox-2 activation in macrophages

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      Vancomycin: ligand recognition, dimerization and super-complex formation (pages 1294–1307)

      ZhiGuang Jia, Megan L. O'Mara, Johannes Zuegg, Matthew A. Cooper and Alan E. Mark

      Version of Record online: 11 FEB 2013 | DOI: 10.1111/febs.12121

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      Molecular dynamics simulations have been used to examine the process of ligand binding and dimerization of vancomycin. The spontaneous formation of vancomycin-ligand complexes, back-to-back and face-to-face dimers was observed. This has allowed a detailed analysis of the origin of the cooperatively between ligand binding and dimerization and suggests that the formation of face-to-face dimers could be functionally significant

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      All-trans retinoic acid protects hepatocellular carcinoma cells against serum-starvation-induced cell death by upregulating collagen 8A2 (pages 1308–1319)

      Wen Wang, Gang Xu, Cui-Ling Ding, Lan-Juan Zhao, Ping Zhao, Hao Ren and Zhong-Tian Qi

      Version of Record online: 6 FEB 2013 | DOI: 10.1111/febs.12122

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      As a therapeutic agent to various cancers, all-trans retinoic acid (atRA) has been reported to inhibit the growth, induce apoptosis or cause differentiation. In this study, atRA was found to protect HCC cells against serum starvation induced cell death, and COL8A2 was identified as the key protein involved in the enhancement of cell adhesion of atRA under serum free condition

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      Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners (pages 1320–1329)

      Sonia Canterini, Valentina Carletti, Stefania Nusca, Franco Mangia and Maria Teresa Fiorenza

      Version of Record online: 31 JAN 2013 | DOI: 10.1111/febs.12123

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      Proteins of TSC22 domain family play isoform-specific roles in cell proliferation, differentiation and apoptosis. We have characterized the nature, subcellular localizations and protein partners of TSC22D4 splice variants TSC22D4-42 and TSC22D4-55 and glyco-phospho-forms TSC22D4-67 and TSC22D4-72. TSC22D4-42 and TSC22D4-67 associate with TSC22D1.2 and mitochondrial AIF, respectively, depending on neuronal differentiation. TSC22D4-72 and TSC22D4-55 are stably chromatin- and nuclear matrix-associated, respectively

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      Caspase-dependent cleavage of the mono-ADP-ribosyltransferase ARTD10 interferes with its pro-apoptotic function (pages 1330–1343)

      Nicolas Herzog, Jörg D. H. Hartkamp, Patricia Verheugd, Fabian Treude, Alexandra H. Forst, Karla L. H. Feijs, Barbara E. Lippok, Elisabeth Kremmer, Henning Kleine and Bernhard Lüscher

      Version of Record online: 13 FEB 2013 | DOI: 10.1111/febs.12124

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      Little is known about the cellular functions of ARTD10/PARP10, a mono-ADP-ribosyltransferase. We found that the ectopic expression of ARTD10, but not a catalytically inactive mutant, induces apoptosis. This is accompanied by caspase-mediated cleavage of ARTD10, which inhibits its proapoptotic function. Moreover DNA damage induced apoptosis is repressed when ARTD10 is knocked-down, suggesting a regulatory role of ARTD10 during apoptosis

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      Structural analysis of the alcohol acyltransferase protein family from Cucumis melo shows that enzyme activity depends on an essential solvent channel (pages 1344–1357)

      Sebastián Galaz, Luis Morales-Quintana, María Alejandra Moya-León and Raúl Herrera

      Version of Record online: 13 FEB 2013 | DOI: 10.1111/febs.12127

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      CmAAT2-ligands complexes show the alcohol located far from the catalytic residues. The longer distance to both catalytic residues contrasts with the shorter distance to A268 in the alternative pocket. In the case of CmAAT1-ligands complex H161 establish a hydrogen bond with the hydroxyl group of acetyl-CoA and D165 establish a hydrogen bond with the hexanol. This explains the differences in the activity for both enzymes

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      Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference (pages 1358–1370)

      Géraldine Buysschaert, Kenneth Verstraete, Savvas N. Savvides and Bjorn Vergauwen

      Version of Record online: 11 FEB 2013 | DOI: 10.1111/febs.12128

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      The crystal structure and cofactor preference of an atypical short chain dehydrogenase (SDR) from Vibrio vulnificus are reported. This SDR lacks the well-conserved catalytic tetrad Asn–Ser–Tyr–Lys as well as the structural motif made up by helix α2 and strand β3, thought to aid in stabilizing the adenosine moiety of the cofactor. This work sets the stage for deriving the identity of the natural substrate of SDRvv and adds a new twist to the structure-function landscape for Rossmann-fold-dependent cofactor discrimination

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      Evidence for self-association of the alternative sigma factor σ54 (pages 1371–1378)

      Massimo Sabbatini, Alessandro Vezzoli, Mario Milani and Giovanni Bertoni

      Version of Record online: 11 FEB 2013 | DOI: 10.1111/febs.12129

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      σ54 was thought to act as monomer in all transcription steps. We provide evidence that σ54 of Pseudomonas putida interacts with itself. The self-self interface amid monomers involves contacts in the σ54 regions I and III. We propose a model of σ54 self-association in tetramers envisaged as “dimer of dimers”. Our results suggest a new auto-antagonistic regulatory state of σ54

  5. Author index

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
    1. You have free access to this content
      Author index (page 1379)

      Version of Record online: 26 FEB 2013 | DOI: 10.1111/febs.8767

  6. Table of Contents

    1. Top of page
    2. Front Cover
    3. Editorial Information
    4. Review Article
    5. Original Articles
    6. Author index
    7. Table of Contents
    1. You have free access to this content
      Table of Contents (page 1380)

      Version of Record online: 26 FEB 2013 | DOI: 10.1111/febs.12195

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