Dual sgRNAs facilitate CRISPR/Cas9-mediated mouse genome targeting (pages 1717–1725)
Jiankui Zhou, Jianying Wang, Bin Shen, Li Chen, Yang Su, Jing Yang, Wensheng Zhang, Xuemei Tian and Xingxu Huang
Article first published online: 26 FEB 2014 | DOI: 10.1111/febs.12735
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) endonuclease, also known as an RNA-guided endonuclease (RGEN), is increasingly being used for eukaryote genome targeting; however, its efficiency, specificity and versatility needs to be optimized. In this issue, Zhou et al. report an improved method for CRISPR/Cas9-mediated gene targeting, and show that the simultaneous use of dual sgRNAs to target an individual mouse gene is a more efficient and specific method than using just a single sgRNA. This new dual sgRNAs strategy will facilitate CRISPR/Cas9-mediated mammalian genome targeting.