Codon optimisation to improve expression of a Mycobacterium avium ssp. paratuberculosis-specific membrane-associated antigen by Lactobacillus salivarius (pages 27–38)
Christopher Johnston, Pierre E. Douarre, Tewfik Soulimane, Daniel Pletzer, Helge Weingart, John MacSharry, Aidan Coffey, Roy D. Sleator and Jim O'Mahony
Version of Record online: 18 APR 2013 | DOI: 10.1111/2049-632X.12040
The manuscript clearly demonstrates that codon optimization improves expression and hence provides a simple but effective way to increase expression levels of the target antigen in a host organism. The underlying approach of expressing Mycobacterium avium paratuberculosis (MAP) genes in Lactobacillus strains is also an interesting idea which has the potential to help immunize cattle against Johne's disease. As this was the first direct assessment of the impact of MAP codon bias on expression in a heterologous host, this study is of importance to the field.