Table S1. Multiplex sets, locus names with fluorescent labels (F = 6-FAM, N  =  NED, P  =  PET, V  =  VIC), primer concentrations, annealing temperatures (TA), and numbers of polymerase chain reaction (PCR) cycles for 16 loci in creek chub.  Reactions were conducted in 15-µL volumes containing 20–40 ng of genomic DNA, 0.5 units of Taq DNA polymerase (Promega), 1X PCR buffer (1.5 mM MgCl2; Promega), and 100 µM of each deoxynucleotide triphosphate. Cycling conditions consisted of 94 ºC for 1 min, denaturation at 94 ºC for 20 s, annealing at TA for 20 s, and extension at 72ºC for 30 s for the designated number of cycles and a final extension at 67 ºC for 45 min. The number of alleles detected (No. Alleles) and the mean number of alleles per site (Mean/Site) are included for the 10 loci used to inform our analyses.

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