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Appendix S1. Supporting information on unpublished studies.

Figure S1. Map of the River Lathkill, indicating the location of the seven sampling site in the study of Stubbington (R. Stubbington, unpubl. data a).

Figure S2. Map of the River Lathkill, indicating the location of the sampling sites in the study of Stubbington (R. Stubbington, unpubl. data b; originally published in Stubbington et al. (2011b) and adapted for reproduction here from the author's original figure).

Figure S3. Map of Chitterne Brook and the River Till, indicating the location of the sampling sites in the study of Wood (P. J. Wood, unpubl. data; original drawing).

Table S1. Invertebrate taxa recorded in the karstic headwaters of the River Lathkill by Stubbington (R. Stubbington, unpubl. data a).

Table S2 Invertebrate taxa recorded the upper reaches of the River Lathkill by Stubbington (R. Stubbington, unpubl. data b).

Table S3 Invertebrate taxa recorded in two chalk streams by Wood (P. J. Wood, unpubl. data).

Table S4 Moisture content (%) recorded in sediments extracted from the karstic headwaters of the River Lathkill by Stubbington (R. Stubbington, unpubl. data a).

Table S5 Grain size distribution of sediments extracted from the karstic headwaters of the River Lathkill by Stubbington (R. Stubbington, unpubl. data a).

Table S7 Grain size distribution of sediments extracted from the upper reaches of the River Lathkill by Stubbington (R. Stubbington, unpubl. data b).

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