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Figure S1. Delta K likelihood plots with regard to the STRUCTURE results in Figure 2 for lake trout sampled in the Kogaluk River catchment in Newfoundland and Labrador, between 2006 and 2011.

Figure S2. The relationship between elevation (meters above sea level) and mean estimates of effective population size of lake trout for each of the 9 unique clusters identified with STRUCTURE found within the Kogaluk River catchment in Newfoundland and Labrador.

Figure S3. The relationship between genetic cluster total lake area and mean estimates of effective population size of lake trout for each of the 9 unique clusters identified with STRUCTURE found within the Kogaluk River catchment in Newfoundland and Labrador.

Figure S4. The relationship between genetic cluster total lake area and mean estimates of effective population size of lake trout for 8 unique clusters, excluding the cluster containing lakes Esker and WP152, identified with STRUCTURE found within the Kogaluk River catchment in Newfoundland and Labrador.

Table S1. Multiplex primer panel composition used for analysis of lake trout in the Kogaluk River catchment in Newfoundland and Labrador, indicating loci dye label, assigned panel number, and source authors.

Table S2. Multiplex and single primer PCR reactions as well as reagent suppliers, used for genetic analysis of lake trout in the Kogaluk River catchment in Newfoundland and Labrador.

Table S3. Multiplex and single primer thermocycle conditions for PCR reactions used for the genetic analysis of lake trout in the Kogaluk River catchment in Newfoundland and Labrador.

Table S4. Average slope measurements for all pairs of lakes sampled for lake trout (between 2006 and 2011) in the Kogaluk River catchment in Newfoundland and Labrador.

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