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gbb12048-sup-0001-tableS1.pdfPDF document29KTable S1: Phenotypic correlations between behavioural traits.
gbb12048-sup-0002-tableS2.pdfPDF document146KTable S2: Analysis of variance for each behavioural phenotype. (a) between the Orco mutant line and its wild-type control, and (b) among the DGRP lines.
gbb12048-sup-0003-tableS3.pdfPDF document89KTable S3: Mean response scores for each behavioural phenotype measured in the DGRP lines.
gbb12048-sup-0004-tableS4.txtplain text document149188KTable S4: Genome wide association results for the 30th percentile phenotype. For each polymorphism, the chromosomal arm and genomic location are indicated. Also shown are the levels of statistical association for male, female, pooled sex and SNP by sex analyses.
gbb12048-sup-0005-tableS5.txtplain text document149191KTable S5: Genome wide association results for the 90th percentile phenotype. For each polymorphism, the chromosomal arm and genomic location are indicated. Also shown are the levels of statistical association for male, female, pooled sex and SNP by sex analyses.
gbb12048-sup-0006-tableS6.txtplain text document149189KTable S6: Genome wide association results for the Intensity phenotype. For each polymorphism, the chromosomal arm and genomic location are indicated. Also shown are the levels of statistical association for male, female, pooled sex and SNP by sex analyses.
gbb12048-sup-0007-tableS7.txtplain text document149188KTable S7: Genome wide association results for the Location ratio phenotype. For each polymorphism, the chromosomal arm and genomic location are indicated. Also shown are the levels of statistical association for male, female, pooled sex and SNP by sex analyses.
gbb12048-sup-0008-tableS8.txtplain text document149188KTable S8: Genome wide association results for the Strength phenotype. For each polymorphism, the chromosomal arm and genomic location are indicated. Also shown are the levels of statistical association for male, female, pooled sex and SNP by sex analyses.
gbb12048-sup-0009-tableS9.xlsxExcel 2007 spreadsheet388KTable S9: Genome-wide association results for all odour-guided phenotypes. Top SNPs associated with each of five odour-guided behavioural phenotypes are listed (P-value < 10−5). For each SNP, its chromosomal location and genomic location are listed. Also listed is the gene that the SNP is associated with, its Flybase ID, and the nature of the SNP. For intergenic SNPs, the proximity (base pairs) to each gene is indicated with separate values indicating the nearest upstream or downstream gene. Also shown are minor allele frequencies for each SNP and P-values indicating the level of significance of the association for female, male, pooled sex and SNP by sex statistical analyses. The effect sizes, major and minor allele calls, the minor and total allele count and the mean sequencing coverage for each SNP across all lines are also listed.
gbb12048-sup-0010-tableS10.xlsxExcel 2007 spreadsheet36KTable S10: Significant enrichment of gene ontology (GO) terms for each odour-guided behaviour phenotype. For each phenotype, significantly enriched (P < 0.05) GO terms are listed for GO categories, Biological Process, Cellular Component, and Molecular Function. The corrected P-values (Benjamini & Hochberg), along with the number of GWA candidate genes and their gene symbols and gene IDs are also listed.
gbb12048-sup-0011-tableS11.xlsxExcel 2007 spreadsheet168KTable S11: Significant differences in olfactory behaviour for genes disrupted in neuronal subpopulations using RNA interference. The individual UAS-RNAi construct and name of the gene disrupted are listed, as are the Gal-4 driver line and the Molecular and Biological processes for each gene. P-values are given when a significant effect on an odour-guided phenotype was found in comparisons between the RNAi mediated knockdown of a gene and its control. A Bonferroni significance threshold of P < 0.008 was used.
gbb12048-sup-0012-figureS1.pdfPDF document73KFigure S1: Histograms for each of the five odour-guided phenotypes. Significant variation exists among the DGRP lines for each phenotype. (a) Intensity, (b) Location ratio, (c) 30th percentile, (d) 90th percentile and (e) Strength.
gbb12048-sup-0013-figureS2.pdfPDF document1810KFigure S2: GWAs and LD plots for SNPs significantly associated with the 30th percentile phenotype. The top panel shows significant GWAs by sex and for the SNP by sex interaction term. The x-axis denotes the major chromosomal arms and the y-axis the level of significance of the association for each SNP. The solid horizontal line denotes a P-value of 10−5 significance threshold. The bottom panel shows the LD plot for SNPs significantly associated with the phenotype. The diagonal represents the R2 value, with a value of 1 indicating complete linkage disequilibrium. The black lines demarcate the major chromosomal arms. Directly above each of these LD plots, the minor allele frequency (top graph) and the significance level (bottom) of the top SNPs associated with a given phenotype are shown.
gbb12048-sup-0014-figureS3.pdfPDF document1810KFigure S3: GWAs and LD plots for SNPs significantly associated with the 90th percentile phenotype. The top panel shows significant GWAs by sex and for the SNP by sex interaction term. The x-axis denotes the major chromosomal arms and the y-axis the level of significance of the association for each SNP. The solid horizontal line denotes a P-value of 10−5 significance threshold. The bottom panel shows the LD plot for SNPs significantly associated with the phenotype. The diagonal represents the R2 value, with a value of 1 indicating complete linkage disequilibrium. The black lines demarcate the major chromosomal arms. Directly above each of these LD plots, the minor allele frequency (top graph) and the significance level (bottom) of the top SNPs associated with a given phenotype are shown.
gbb12048-sup-0015-figureS4.pdfPDF document1810KFigure S4: GWAs and LD plots for SNPs significantly associated with the Intensity phenotype. The top panel shows significant GWAs by sex and for the SNP by sex interaction term. The x-axis denotes the major chromosomal arms and the y-axis the level of significance of the association for each SNP. The solid horizontal line denotes a P-value of 10−5 significance threshold. The bottom panel shows the LD plot for SNPs significantly associated with the phenotype. The diagonal represents the R2 value, with a value of 1 indicating complete linkage disequilibrium. The black lines demarcate the major chromosomal arms. Directly above each of these LD plots, the minor allele frequency (top graph) and the significance level (bottom) of the top SNPs associated with a given phenotype are shown.
gbb12048-sup-0016-figureS5.pdfPDF document1810KFigure S5: GWAs and LD plots for SNPs significantly associated with the Location ratio phenotype. The top panel shows significant GWAs by sex and for the SNP by sex interaction term. The x-axis denotes the major chromosomal arms and the y-axis the level of significance of the association for each SNP. The solid horizontal line denotes a P-value of 10−5 significance threshold. The bottom panel shows the LD plot for SNPs significantly associated with the phenotype. The diagonal represents the R2 value, with a value of 1 indicating complete linkage disequilibrium. The black lines demarcate the major chromosomal arms. Directly above each of these LD plots, the minor allele frequency (top graph) and the significance level (bottom) of the top SNPs associated with a given phenotype are shown.
gbb12048-sup-0017-figureS6.pdfPDF document1810KFigure S6: GWAs and LD plots for SNPs significantly associated with the Strength phenotype. The top panel shows significant GWAs by sex and for the SNP by sex interaction term. The x-axis denotes the major chromosomal arms and the y-axis the level of significance of the association for each SNP. The solid horizontal line denotes a P-value of 10−5 significance threshold. The bottom panel shows the LD plot for SNPs significantly associated with the phenotype. The diagonal represents the R2 value, with a value of 1 indicating complete linkage disequilibrium. The black lines demarcate the major chromosomal arms. Directly above each of these LD plots, the minor allele frequency (top graph) and the significance level (bottom) of the top SNPs associated with a given phenotype are shown.
gbb12048-sup-0018-figureS7.pdfPDF document18KFigure S7: Quantile–Quantile plots for each of the five odour-guided phenotypes. (a) Intensity (b) Location ratio (c) 30th percentile (d) 90th percentile, and (e) Strength. The different symbols indicate P-values for the SNP term in the full model (grey squares), the SNP by sex interaction term in the full model (light grey circles), the model run with female only (black triangles), and with male only (grey diamonds).
gbb12048-sup-0019-figureS8.pdfPDF document4942KFigure S8: Genetic network of GWA candidate genes significantly associated with each of five odour-guided phenotypes. (a) Network of candidate genes in which the green circles denote each phenotype, with circle size a reflection of the number of candidate gene connections. Blue and purple circles represent single GWA candidate genes, with purple denoting genes with known neural function. (b) Networks denote GWA candidate genes with known genetic and/or protein–protein interactions. The circle size reflects the number of connections for a given GWA candidate gene. Genes with known neural function are shown as purple circles. All other genes are shown as blue.
gbb12048-sup-0020-figureS9.pdfPDF document5KFigure S9: Network of significantly enriched molecular function gene ontology (GO) terms for odour-guided behaviour. GWAs for each phenotype were placed into Molecular function GO categories. GWAs for five odour-guided phenotypes (green squares) showed significant enrichment for specific GO terms (hexagons). GO terms are colour coded to reflect uniqueness to a single phenotype (yellow) or being shared among phenotypes (peach). GO terms involved in nervous system function were irrespective of their connections coloured purple.

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