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Fig. S1 Raw calorimetric data of (A) Nitrosospira briensis from pH 10.2 to 3.6, (B) Nitrosomonas europaea from pH 10.0 to 3.8, (C) Nitrosolobus multiformis from pH 9.9 to 4.4, (D) Nitrosococcus oceani from pH 10.3 to 5.8, (E) Nitrosopumilus maritimus strain SCM1 from pH 9.4 to 4.6, and (F) Sulfolobus acidocaldarius from pH 9.0 to 3.5.

Fig. S2 Cumulative heats of methylphosphonate protonation versus total protons added.

Table S1 Methylphosphonic acid thermodynamic parameters.

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