gbi12082-sup-0001-FigS1.epsimage/eps7381KFig. S1 Descriptions and diversity of sample set with depth. Sample type is coded by shape: triangles, microbialite samples; circles, nodule samples; diamonds, soft mat samples. Checkered samples were Sanger sequenced with Cyanobacteria-specific primers. General bacteria primers were used for nodules. The calculated chao1 diversity estimates of pyrosequenced data from corresponding depths are graphed. Photographs show different microbialite morphotypes at various depths. Black and white bars in photos from 85 feet and 106 feet are 10 cm. Photo credit: Donnie Reid.
gbi12082-sup-0002-FigS2.epsimage/eps1600KFig. S2 Phylogenetic tree of abundant OTUs from pyrosequencing of microbialites and Sanger sequencing of nodules with general bacteria primers. Shown are Cyanobacteria-related OTUs from microbialites (green), OTUs from nodule analysis (purple), Non-Cyanobacteria OTUs from microbialites (red). The tree was constructed using neighbor-joining method with 1000 bootstrap replicates. Bootstrap values over 60% are shown.
gbi12082-sup-0003-FigS3.pdfapplication/PDF35KFig. S3 The top 50 most abundant OTUs from microbialites and soft mats via 454 pyrosequencing and their abundance within the dataset. OTUs shaded in green are Cyanobacteria-related. OTUs marked with a red star are from subsection-III within the Cyanobacteria phylum in the silva database. Only six of 50 OTUs are shared (OTU-7552, OTU-11499, OTU-11510, OTU-2606, OTU-5406, OTU-12002).

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