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gcb12003-sup-0001-AppendixS1-TableS1.docWord document32KAppendix S1. Comparison of experimental methodologies. Table S1. The ice nucleation temperatures of B. tectorum accession R1 grown at 12/10 °C.
gcb12003-sup-0002-FigureS1.pdfapplication/PDF57KFigure S1. The response of electrolyte leakage to low exposure temperature in leaf samples of Bromus tectorum and B. rubens. Samples were frozen at 4.5 °C h−1 without the presence of water/ice (a), with an addition of water (b) and ice crystals (c).
gcb12003-sup-0003-FigureS2.pdfapplication/PDF133KFigure S2. The response of electrolyte leakage to low exposure temperature in leaf samples of Bromus tectorum and B. rubens. Samples were frozen at 2 °C h−1 (a), 4.5 °C h−1 (b) and 6 °C h−1 (c).
gcb12003-sup-0004-FigureS3.pdfapplication/PDF134KFigure S3. (a) A representative response of Fv/Fm 24 h after the freezing cycle in leaf samples of Bromus tectorum T1 and B. rubens R1. (b) The relationship between the temperature causing 50% reduction in Fv/Fm measured in the dark 24 h after the freezing cycle and the TEL50 in B. tectorum and B. rubens.
gcb12003-sup-0005-FigureS4.pdfapplication/PDF214KFigure S4. Results of the freezing tests of 2009–2010, as a function of the day of year for Bromus tectorum accession T1, B. tectorum accession T5 and B. rubens accession R1.
gcb12003-sup-0006-FigureS5.pdfapplication/PDF16KFigure S5. A representative freezing curve for Bromus tectorum showing the exotherm at the ice nucleation point.

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