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gcb12109-sup-0001-FigureS1.tifimage/tif2151KFigure S1. Total organic content (TOC = shell free dry mass (SFDM) + OSC, black) and organic shell component (OSC = shell matrix proteins and carbohydrates + periostracum, white) of settled M. edulis as a function of shell length. TOC = 0.018 (±0.003) * shell length (mm)2.705 (±0.125), F(1,84) = 959.4, P < 0.01; OSC = 0.009 (±0.002) * shell length (mm)2.735 (±0.127), F(1,82) = 987.2, P < 0.01; Values represent means ± SE.
gcb12109-sup-0002-FigureS2.tifimage/tif2439KFigure S2. Energetic content (J) of the CaCO3 mass (filled bars) and total organic mass (SFDM+OSC, striped bars) of settled M. edulis larvae. Calculations were performed by converting the masses shown in Fig. 1d into energy equivalents using values of 2 J mg−1 for CaCO3 and 23 J mg−1 for organic mass (Brey et al. 1988, Palmer 1992). n = 7. Bars represent means ± SD.
gcb12109-sup-0003-FigureS3.tifimage/tif2296KFigure S3. Bi-weekly measured shell length of settled M. edulis sampled during the field experiment in 2010, inner fjord (black), outer fjord (grey). Bars represent means ± SD.
gcb12109-sup-0004-FigureS4.tifimage/tif8275KFigure S4. Coverage (a) and CaCO3 production (b) of M. edulis (black) and A. improvisus (grey) on settlement panels at stations OF and IF. Bars represent means ± SD.
gcb12109-sup-0005-FigureS5.tifimage/tif6411KFigure S5. Comparison of M. edulis acid–base status of haemolymph sampled (I) immediately after removal of the animal from the fjord or (II) after 5–10 min storage of specimens in ambient seawater (a–c), Change of measured pH with time after an initial stable value has been reached about 30 s after beginning of the measurement, no significant effect of CO2 out gassing on pH is observed during the first 3 min (d). Davenport diagram of M. edulis haemolymph acid–base status. Specimens were kept for 7 days at two pCO2 levels (a+b: 380 μatm; c+d: 4000 μatm) and two flow rates (a+c: 100 ml min−1; b+d: 0 ml min−1), means ± SD, n = 5. Specimens at 4000 μatm and 0 ml min−1 (d) had significant higher HCO3 levels than the other groups (ANOVA, F(3,16) = 11.4, P < 0.01) (e).
gcb12109-sup-0006-TableS1-S8.docWord document185K

Table S1. Energy supply settings and weekly shell length growth during the laboratory experiment. Data are mean values of all pCO2 treatments per feeding level. Shell length growth was calculated from weekly measurements and pooled for all pCO2 treatments of one feeding level. Values represent means ± SD.

Table S2. Carbonate system speciation during the laboratory experiment, mean salinity = 14.2 ± 0.9, mean temperature = 17.2 ± 0.2 °C. Values represent means ± SD.

Table S3. Multiple regression of observed growth of shell length, inorganic shell component (ISC) and total organic (TOC) means ± SE, A; Comparison of growth model using AIC, B.

Table S4. Relative contribution of shell free drymass (SFDM) and shell components [organic shell component (OSC) + inorganic shell component (ISC)] to total mass (A) and energy content (B) of settled M. edulis in the four pCO2 treatments.

Table S5. Shell mass, organic shell component (OSC) and OSC in % of shell mass shell of juvenile M. edulis (shell length 12.4 ± 1.1 mm) from Kiel Fjord including mean ± SD.

Table S6. Location of the carbonate system monitoring stations and sampling depths, mean salinity, temperature, pH, pCO2, Ω, PO43− and SiO3 measured in surface and bottom water samples during monitoring 2009/10. Values represent means ± SD.

Table S7. Haemolymph acid-base status of M. edulis from the inner fjord measured in 2009 at ambient seawater temperature and salinity, n = 5 at each time point. Values represent means ± SD.

Table S8. Carbonate system speciation at two pCO2 levels and two flow rates (0 and 100 mL min−1), mean salinity = 17.6 ± 0.3, mean temperature = 11.1 ± 1.1 °C.

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