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gcb12197-sup-0001-FigS1.docWord document258KFigure S1. Survival curves of L. elliptica incubated under normoxic, hypoxic (2% O2) and anoxic (0% O2) conditions. LT50: 10 days for anoxia and 17 days for hypoxia. N = 6 for anoxia, 8 animals for hypoxia and 12 for normoxia.
gcb12197-sup-0002-FigS2.docWord document141KFigure S2. Apoptotic activity in gill tissue of older L. elliptica individuals incubated for 16 days under normoxic (16 days N) or hypoxic (16 days H; 2% O2) conditions and siphon tissue of older and younger individuals under the same treatment. *indicate significant differences (P < 0.05, Mann–Witney t-test). N = 8–12 per group.
gcb12197-sup-0003-FigS3.pptapplication/ppt106KFigure S3. Q-PCR results showing relative gene expression in siphon tissue. A: younger vs. older hypoxic animals, with up-regulation in young animals of all genes tested. B: Older hypoxic vs. older normoxic animals, with the genes being up-regulated in hypoxic animals
gcb12197-sup-0004-TableS1.docWord document37KTable S1. Primer sequences used in Q-PCR validation.
gcb12197-sup-0005-TableS2.docWord document52KTable S2. Adenylate concentrations (μmol × gram wet weight) and energy charge (EC) in mantle, siphon and gill tissue of young and old L. elliptica control individuals. Different letters between tissues within one age group and parameter mark significant differences (nonparametric one-way anova, P < 0.05). N = 6 (young individuals) or 4 (old individuals). * marks differences between young and old individuals of the hypoxia experiment within one parameter (Mann–Whitney U-test). The ATP : AMP ratios are also given, these are calculated from the mean values.
gcb12197-sup-0006-TableS3.docWord document63KTable S3. Adenylate concentrations (μmol × gram wet weight) in mantle, siphon and gill tissue of young and old L. elliptica individuals incubated for 16 days under normoxic (16 days N) or hypoxic (16 days H; 2% O2) conditions. Different letters between treatments within one age group mark significant differences (nonparametric one-way anova; P < 0.05).
gcb12197-sup-0007-TableS4.docWord document99KTable S4. Nicotinamide nucleotide concentration (nmol × gramwet weight) and ratios (NAD/NADH; NADP/NADPH) in the mantle, siphon and gill tissue of young and old L. elliptica individuals incubated under normoxic, hypoxic (2% O2) and anoxic conditions.
gcb12197-sup-0008-TableS5.docWord document30KTable S5. Changes in HSP expression in gill tissue of younger and older individuals incubated for 16 days under hypoxic or normoxic condition. N = 6 per group.
gcb12197-sup-0009-TableS6.docWord document40KTable S6 Clones with putatively ascribed functions identified in gill tissue from older animals under hypoxic conditions.
gcb12197-sup-0010-TableS7.docWord document39KTable S7. Clones with putatively ascribed functions identified in gill tissue from young animals under hypoxic conditions.
gcb12197-sup-0011-TableS8.docWord document36KTable S8. Clones with putatively ascribed functions identified in siphon tissue from young animals under hypoxic conditions.
gcb12197-sup-0012-TableS9.docWord document35KTable S9. Regression data for the shell and tissue scaling in L. elliptica. All measurements were converted to natural logs and compared with Ln_length.

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