gcb12227-sup-0001-FigS1-S15_TableS1-3.docxWord document5106K

Figure S1. Lateral front view of the experimental life support system.

Figure S2. Water and tide circulation system detail (Front). A, independent microcosm; B, saltwater reservoir; C, acidified saltwater tide reservoir; D, normal pH saltwater tide reservoir; E, inlet pipe (to return the exceeding water to the tide reservoir); F, Outlet pipe (to discharge the microcosm waste water after tide circulation).

Figure S3. Water and tide circulation system detail (Back). A, outlet pipe from the microcosm; B, inlet pipe in the microcosm; C, inflow water pump.

Figure S4. Water and tide circulation system detail (top view). A, Outflow pump positioned inside a PVC cylinder and protected with a mesh screen.

Figure S5. pH control system detail: A, CO2 bottle; B, solenoid valve; C, pH controller; D, pH electrode probe.

Figure S6. Water bath detail (from back). A, waterbath (the 2 tanks were drilled in the bottom and connected through a 40 mm PVC pipe); B, individual microcosm; C, refrigerator; D, canister filter pump; E, Stainless Steel 118 structure 40 × 2 mm.

Figure S7. Lighting system detail: A, luminaire.

Figure S8. Luminaire detail: A, daylight and ultraviolet lamps dispose alternately.

Figure S9. Experimental light spectra. A, Spectrum of photosynthetically active radiation (PAR: 400–700 nm) of the fluorescent tubes set to 100% intensity.

Figure S10. Average pH measured during 1 week every 2 days in control and reduced pH treatments.

Figure S11. Concentration of dissolved inorganic nutrients (nitrate NO3; ammonium NH4+ and o-phosphate PO43−) in water ELSS control at the beginning of the experiment, after 21 days and at the end of the experiment.

Figure S12. Principal coordinates analysis of Denaturing-gradient gel electrophoresis fingerprints of 16S rRNA gene fragments amplified at time 0 (ConBs), 21 days (ConIs) and 57 days (ConFs).

Figure S13. Rarefied OTU richness as a function of the number of sequences from ConBs (environment) and ConFs (microcosm) samples.

Figure S14. Principal coordinates analysis of operational taxonomic unit (OTU) composition.

Figure S15. Heatmap showing the abundance of dominant 16S rRNA cDNA sequence reads (≥50 sequences).

Table S1. Artificial life support system main components, their manufacturers and suppliers.

Table S2. Diurnal cycles of the PAR and UV integrated radiation intensities expressed as percentages of maxima intensities of the respective fluorescence tubes.

Table S3. The environmental range of water parameters (temperature, salinity and pH), and of dissolved inorganic nutrients (nitrate NO3, ammonium NH4+ and o-phosphate PO43−) in Portuguese coastal systems and in the ELSS microcosms (control experimental units).


Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.