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gcb12251-sup-0001-FigS1.pdfapplication/PDF282KFig. S1 Cross design modified from Kelly & Hofmann, 2012. We collected adult purple urchins (Strongylocentrotus purpuratus), from populations at two sites in California, USA (inset), thought to differ in their carbonate chemistry regimes. In the laboratory, we crossed each male with two females from his own sites, and two females from the opposite site (N = 8 males/site, 15 females/sites), and then spilt embryos from each cross into high- and low-pCO2 rearing conditions.
gcb12251-sup-0002-FigS2.pdfapplication/PDF936KFig. S2 Projected future larval body length and change in density-independent growth rate (r) under high-pCO2 conditions across a range of values for selection on body size (γ), additive genetic variance for body size (σA2), and number of generations to reach pH = 7.6 (i). Model outcomes are similar values of i ranging from 5 to 20.
gcb12251-sup-0003-FigS3.pdfapplication/PDF44KFig. S3 Larval respiration rates (pmol O2/hr/larva) for offspring of parents from two sites reared under high and low pCO2. Two-way anova revealed no effect of site (F1,20 = 0.41, P = 0.53) pCO2 level (F1,20 = 0.02, P = 0.90) or pCO2 X site interaction (F1,20 = 0.13, P = 0.72) on respiration rate.
gcb12251-sup-0004-FigS4.pdfapplication/PDF93KFig. S4 Hypothetical box plots showing the distribution of larval sizes for four sires under high and low pCO2. In (a), variation in size under high pCO2 is driven by variation among families in overall size. In (b), variation in size under high pCO2 is driven by variation among families in sensitivity of growth to high pCO2.
gcb12251-sup-0005-TableS1.pdfapplication/PDF59KTable S1. Cross design.
gcb12251-sup-0006-TableS2.pdfapplication/PDF80KTable S2. Water chemistry and carbonate parameters for larval culturing. pH total alkalinity (TA), salinity, and temperature were the measured parameters, and the remaining parameters were calculated in CO2SYS. pH was measured in each larval culture vessel each day of the experiment, TA was measured in each of the mixing reservoirs for each day of the experiment. Mean values ± SD are shown for each week of the experiment.

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