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Table S1. Matrix of Pearson product-moment correlations among environmental predictor variables (all except pH log10 transformed).

Table S2. Water quality at sites sampled in the Blackwood River.

Table S3. Prevalence of species and the water quality at their capture sites in the Blackwood River.

Figure S1. Mean number of Galaxias occidentalis moving in the tributary and main channel sites of the Blackwood River. NF indicates that the system was not flowing and therefore was not sampled.

Figure S2. Length-frequency distributions of Galaxias occidentalis in Milyeannup Brook.

Figure S3. Length-frequency distributions of Galaxias occidentalis in Rosa Brook.

Figure S4. Mean number of Galaxiella munda moving in the tributary sites of the Blackwood River. NF indicates that the system was not flowing and therefore was not sampled.

Figure S5. Length-frequency distributions of Galaxiella munda in Milyeannup Brook.

Figure S6. Mean number of Nannatherina balstoni moving in the tributary and main channel sites of the Blackwood River. NF indicates that the system was not flowing and therefore was not sampled.

Figure S7. Length-frequency distributions of Nannatherina balstoni in Milyeannup Brook.

Figure S8. Mean number of Nannoperca vittata moving in the tributary and main channel sites of the Blackwood River. NF indicates that the system was not flowing and therefore was not sampled.

Figure S9. Length-frequency distributions of Nannoperca vittata in Milyeannup Brook.

Figure S10. Length-frequency distributions of Nannoperca vittata in Rosa Brook.

Figure S11. Mean number of Bostockia porosa moving in the tributary and main channel sites of the Blackwood River. NF indicates that the system was not flowing and therefore was not sampled.

Figure S12. Length-frequency distributions of Bostockia porosa in Rosa Brook.

Figure S13. Length-frequency distributions of Bostockia porosa in Layman Brook.

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