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geb12070-sup-0001-fs1.pdf265K

Figure S1 Secondary multivariate models that predict patterns of CMic and CMic/COrg. (A) Observed and predicted soil profile CMic estimates from Model-2: slope = 1.0 (± 5.9 × 10−2, β ± 1SEM), intercept: −1.6 × 10−8 (± 1.2 × 10−2), R2 = 43.9%, F1–365 = 285; P < 0.0001. (B) Observed and predicted topsoil CMic/COrg ratios from Model-3: Slope = 1.0(± 5.2 × 10−2; β ± 1SEM), intercept: −1.8 × 10−5, R2 = 49.9%, F1–365 = 363; P < 0.0001. Shadowed area is the 95% C.I and all axes are in log10 scale. Panels (C) and (D) are the relative importance of regressors included in Model 2 and 3, respectively. In both, panels equal lower-case letter indicate that confidence intervals for the difference between the contributions of each regressor includes zero (P < 0.05). Also, metrics are normalized to sum 100% of explained variance and 95% C.I. were obtained after 1000 bootstrap runs. Legend and biome classification as follows: boreal forest (□, BF), desert (○, D), tundra (▵, T), temperate coniferous forest (+, TCF), temperate deciduous forest (×, TDF), tropical forest (◊, TF), and temperate grassland (▿, TG). MAP: mean annual precipitation; ETo: annual evaporative demand; MAT: mean annual temperature; MaxWD: maximum monthly frequencies of wet days; Total N: soil total nitrogen content; CEC: soil cation exchange capacity; pH: soil pH; C-N ratio: soil Carbon-Nitrogen ratio.

geb12070-sup-0002-fs2.pdf257K

Figure S2 Relationship between topsoil total nitrogen and CMic/COrg. Biome category is presented on top of each panel, label is as follows: (BF) boreal forest, (D) desert, (T) tundra, (TCF) temperate coniferous forest, (TDF) temperate deciduous forest, (TF) tropical forest and (TG) temperate grassland. Regression coefficients and standard error (β ± 1SEM) from the corresponding bivariate regressions are as follows: BF: −0.79(± 0.23), P < 0.001; D: +1.22(± 0.44), P < 0.001; T: −0.58(± 0.13), P < 0.0001; TCF: −0.40(± 0.41), P > 0.05; TDF: −1.14(± 0.13), P < 0.0001; TF: −0.38(± 0.13), P < 0.001; TG: −0.76(± 0.14), P < 0.0001. In all graphs, grey lines represent the bivariate regression line and the shaded-area the 95% C.I.

geb12070-sup-0003-ts1.pdf108K

Table S1 Environmental drivers of soil profile microbial biomass carbon (g CMic m−2). Bivariate regressions of annual and seasonal climatic variables versus microbial biomass.

geb12070-sup-0004-as1.docx1565K

Appendix S1 Supplementary information on materials, methods, and results.

geb12070-sup-0005-as2.pdf400K

Appendix S2 Scatterplots and Person's correlation factors of both soil microbial biomass carbon (CMic) and soil microbial biomass carbon-soil organic carbon (CMic/COrg), and environmental variables.

geb12070-sup-0006-as3.zip191K

Appendix S3 ASCII maps of soil profile microbial biomass carbon (CMic), and for the topsoil soil microbial biomass carbon-soil organic carbon (CMic/COrg).

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