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Figure S1 Global distribution of the non-indigenous species found in the present study.

Figure S2 Haplotype frequencies for each site and species.

Figure S3 Embryonic development and larval development at different temperatures.

Figure S4 Settlement and metamorphosis rates across temperature treatments and between days.

Figure S5 Time series of mean seawater surface temperature recordings.

Figure S6 Time series using the largest difference between maximum and minimum annual temperatures.

Figure S7 Shipping intensity estimated indirectly from the temperature records.

Table S1 Name and genetic characteristics of the sampled sites.

Table S2 GenBank accession numbers for all the sequences included in our study.

Table S3 Primers (5′–3′) used for each of the studied species.

Table S4 Sample sizes of seawater temperature recordings for each site and year.

Table S5 Species found during the field samplings considering species status.

Table S6 Models testing the effects of temperature on settlement and metamorphosis.

Appendix S1 Biogeographic provinces found along the South African coastline.

Appendix S2 Methodology used in the field surveys and species identification.

Appendix S3 Species used for the genetic analyses.

Appendix S4 Genetic methods. DNA extraction, amplification and sequencing.

Appendix S5 Sampling sites and general field methodology.

Appendix S6 Larval culture methods and experimental methodology.

Appendix S7 Methodology used to obtain seawater temperature recordings.

Appendix S8 Interpretation of the genetic patterns per species.

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