Candida albicans adhesion on reinforced polymethylmethacrylate denture resin: effect of fibre architecture and exposure to saliva

Authors

  • Buket Akalın-Evren,

    Corresponding author
    1. Department of Prosthodontics, Faculty of Dentistry, University of Marmara, Istanbul, Turkey
    • Corresponding to:

      Dr Buket Akalın-Evren, Faculty of Dentistry, Department of Prosthodontics, University of Marmara, Güzelbahçe Büyükçiftlik Sok. No:6, 80200 Nisantası, Istanbul, Turkey.

      Tel: +90 0 212 2319120

      Fax: +90 0 212 2465247

      E-mail: buketakalin@hotmail.com

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  • Yasemin Kulak-Özkan,

    1. Department of Prosthodontics, Faculty of Dentistry, University of Marmara, Istanbul, Turkey
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  • Mutlu Özcan,

    1. Center for Dental and Oral Medicine, Clinic for Fixed and Removable Prosthodontics and Dental Materials Science, Dental Materials Unit, University of Zurich, Zurich, Switzerland
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  • Tanju Kadir

    1. Department of Microbiology, Faculty of Dentistry, University of Marmara, Istanbul, Turkey
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Abstract

Background and objectives

Fiber-reinforced composites (FRC) are used to reinforce the prosthetic and restorative appliances. This may result in the exposure of the FRCs which may affect the adherence of microorganisms. This study evaluated the adhesion of Candida albicans to a denture base resin (= 48), reinforced with E-glass FRCs with different architectures [unidirectional (n = 16) and woven (n = 16)], when exposed to either saliva or distilled water.

Materials and methods

Specimens without FRC reinforcement served as control (n = 16). After fiber surfaces were exposed, half of the specimens (n = 8/per group) were treated with saliva, the other half was stored in distilled water prior to C. albicans adhesion. The adhered microorganisms were counted under an optical microscope and the data were analyzed.

Results

When exposed to distilled water or saliva, specimens with unidirectional (5403.4 cells/cm2 and 5013.4 cells/cm2, respectively) (p = 0.202), woven fibers (4053.5 cells/cm2 and 3726.6 cells/cm2, respectively) (= 0.283) and specimens without fibers (2250 cells/cm2 and 2006.8 cells/cm2, respectively) (p = 0.423) showed C. albicans adhesion, being not significant. In general, all the specimens exposed to saliva showed 3582.2 cells/cm2 C. albicans adhesion, while specimens exposed to distilled water showed 3902 cells/cm2 C. albicans adhesion, yet being not significant (= 0.436). Regarding fiber type, C. albicans adhesion was significantly affected by the exposed FRC architecture with more favorable results for woven fibers (p < 0.001).

Conclusion

Polymethylmethacrylate without FRCs collected less C. albicans. The presence of saliva seems to reduce the adhesion.

Ancillary