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Figure S1 Survival rates of yeast cells after each stress treatment. Cells were treated with mild stress (37˚C or 5% ethanol) or the combined stress (CS) directly or after the pretreatment with mild stress. After treatment with each stress for 30 min, cells were diluted with 10 mM potassium phosphate buffer (pH 6.8) and spread on YPD plates (2% glucose, 2% peptone, 1% yeast extract, and 2% agar, pH 5.5). Viable cell numbers were determined by counting colonies after incubation at 28˚C for 2 days. The viability of cells before stress treatment was taken as 100%. The experiments were repeated three times. Data are represented as means ± SEM.

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