Communicated by: Hiroji Aiba
Adaptive response in stress granule formation and bulk translational repression upon a combined stress of mild heat shock and mild ethanol stress in yeast
Article first published online: 30 AUG 2013
© 2013 The Authors Genes to Cells © 2013 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd
Genes to Cells
Volume 18, Issue 11, pages 974–984, November 2013
How to Cite
Yamamoto, Y. and Izawa, S. (2013), Adaptive response in stress granule formation and bulk translational repression upon a combined stress of mild heat shock and mild ethanol stress in yeast. Genes to Cells, 18: 974–984. doi: 10.1111/gtc.12090
- Issue published online: 28 OCT 2013
- Article first published online: 30 AUG 2013
- Manuscript Accepted: 11 JUL 2013
- Manuscript Received: 14 MAY 2013
Figure S1 Survival rates of yeast cells after each stress treatment. Cells were treated with mild stress (37˚C or 5% ethanol) or the combined stress (CS) directly or after the pretreatment with mild stress. After treatment with each stress for 30 min, cells were diluted with 10 mM potassium phosphate buffer (pH 6.8) and spread on YPD plates (2% glucose, 2% peptone, 1% yeast extract, and 2% agar, pH 5.5). Viable cell numbers were determined by counting colonies after incubation at 28˚C for 2 days. The viability of cells before stress treatment was taken as 100%. The experiments were repeated three times. Data are represented as means ± SEM.
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