Figure S1 Domain structures of mammalian ORP4 and oxysterol-binding protein.

Figure S2 ORP family members in H. sapiens, Drosophila melanogaster and Caenorhabditis elegans.

Figure S3 Sequence alignments of members in ORP subfamily I.

Figure S4 A flowchart summarizing procedures for the isolation of Sertoli cells from germ cell-rich fraction.

Figure S5 In vitro fertilization experiments with zona pellucida-intact eggs from wild-type females and sperm from ORP4+/+ and ORP4−/− males.

Figure S6 (A) Immunohistochemical detection of mouse sperm protein sp56 (green) which is localized in the acrosomal matrix. (B) Transverse sections through midpiece of ORP4+/+and ORP4−/− sperm.

Figure S7 A photo of pups grown normally after intracytoplasmic sperm injection with ORP homozygotic sperm into oocyte.

gtc12105-sup-0002-MovieS1.movvideo/mov6378KMovie S1 ORP4+/+ epididymal spermatozoa after 30-min incubation in human tubal fluid (HTF) medium at 37°C.
gtc12105-sup-0003-MovieS2.movvideo/mov7279KMovie S2 ORP4−/− epididymal spermatozoa after 30-min incubation in HTF medium at 37°C.

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