Communicated by: Masayuki Miura
Oligo-astheno-teratozoospermia in mice lacking ORP4, a sterol-binding protein in the OSBP-related protein family
Version of Record online: 19 NOV 2013
© 2013 The Authors Genes to Cells © 2013 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd
Genes to Cells
Volume 19, Issue 1, pages 13–27, January 2014
How to Cite
Udagawa, O., Ito, C., Ogonuki, N., Sato, H., Lee, S., Tripvanuntakul, P., Ichi, I., Uchida, Y., Nishimura, T., Murakami, M., Ogura, A., Inoue, T., Toshimori, K. and Arai, H. (2014), Oligo-astheno-teratozoospermia in mice lacking ORP4, a sterol-binding protein in the OSBP-related protein family. Genes to Cells, 19: 13–27. doi: 10.1111/gtc.12105
- Issue online: 25 DEC 2013
- Version of Record online: 19 NOV 2013
- Manuscript Accepted: 19 SEP 2013
- Manuscript Received: 28 AUG 2013
Figure S1 Domain structures of mammalian ORP4 and oxysterol-binding protein.
Figure S2 ORP family members in H. sapiens, Drosophila melanogaster and Caenorhabditis elegans.
Figure S3 Sequence alignments of members in ORP subfamily I.
Figure S4 A flowchart summarizing procedures for the isolation of Sertoli cells from germ cell-rich fraction.
Figure S5 In vitro fertilization experiments with zona pellucida-intact eggs from wild-type females and sperm from ORP4+/+ and ORP4−/− males.
Figure S6 (A) Immunohistochemical detection of mouse sperm protein sp56 (green) which is localized in the acrosomal matrix. (B) Transverse sections through midpiece of ORP4+/+and ORP4−/− sperm.
Figure S7 A photo of pups grown normally after intracytoplasmic sperm injection with ORP homozygotic sperm into oocyte.
|gtc12105-sup-0002-MovieS1.mov||video/mov||6378K||Movie S1 ORP4+/+ epididymal spermatozoa after 30-min incubation in human tubal fluid (HTF) medium at 37°C.|
|gtc12105-sup-0003-MovieS2.mov||video/mov||7279K||Movie S2 ORP4−/− epididymal spermatozoa after 30-min incubation in HTF medium at 37°C.|
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