Toll-Like Receptors and Cytokines are Upregulated during Helicobacter pylori Infection in Children

Authors

  • Hugo Lagunes-Servin,

    1. Unidad de Investigación Médica en Enfermedades Infecciosas, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Javier Torres,

    1. Unidad de Investigación Médica en Enfermedades Infecciosas, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Carmen Maldonado-Bernal,

    1. Unidad de Investigación en Enfermedades Oncológicas, Hospital Infantil de México Federico Gómez, SS, Mexico City, México
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  • Martha Pérez-Rodríguez,

    1. Unidad de Investigación Médica en Inmunología, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Sara Huerta-Yépez,

    1. Unidad de Investigación en Enfermedades Oncológicas, Hospital Infantil de México Federico Gómez, SS, Mexico City, México
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  • Armando Madrazo de la Garza,

    1. Departamento de Gastroenterología y Endoscopia, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Leopoldo Muñoz-Pérez,

    1. Unidad de Investigación Médica en Enfermedades Infecciosas, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Lourdes Flores-Luna,

    1. Centro de Investigación en Salud Poblacional, Instituto Nacional de Salud Pública, Cuernavaca, México
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  • Guillermo Ramón-García,

    1. Departamento de Anatomía Patológica, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
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  • Margarita Camorlinga-Ponce

    Corresponding author
    1. Unidad de Investigación Médica en Enfermedades Infecciosas, Centro Médico Nacional Siglo XXI, IMSS, Mexico City, México
    • Reprint requests to: Margarita Camorlinga-Ponce, UIMEIP, Hospital de Pediatría CMN Siglo XXI Av. Cuauhtémoc 330, 2°P. Col Doctores, México City CP 06720, México.

      E-mail: margaritacamorlinga@yahoo.com

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Abstract

Background and Aim

Helicobacter pylori infection is mainly acquired during childhood, and establishes a chronic infection that may lead to peptic ulcer or gastric cancer during adulthood. Toll-like receptors (TLRs) are expressed by distinct cell types throughout the gastrointestinal tract, and play an important role in regulation of the innate immune response. Few works have addressed TLRs expression in gastric epithelia of adults, and scarce studies have done it in children. The aim of this work was to analyze the expression of TLR2, TLR4, TLR5, TLR9, and IL-8, IL-10 and TNF-α in the gastric mucosa of children with and without H. pylori infection.

Methods

Gastric biopsies were collected by endoscopy from 50 children with recurrent abdominal pain, 25 with H. pylori infection and 25 without infection. In the gastric biopsies the expression of TLRs and cytokines was studied by immunohistochemistry, and the degree of mucosal inflammation was determined using the Sydney system.

Results

We found that H. pylori infection was associated with a significant increased expression of TLRs 2, 4, 5 and 9, although expression varied between surface epithelia and glands. Epithelial cells expressing IL-8, IL-10 and TNF-α were increased in gastric mucosa of children with H. pylori infection.

Conclusion

This study shows the gastric epithelia of children respond to H. pylori infection by increasing the expression of TLR2, TLR4, TLR5, TLR9 and the cytokines IL-8, IL-10 and TNF-α.

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