Toll-Like Receptors and Cytokines are Upregulated during Helicobacter pylori Infection in Children
Article first published online: 22 JUL 2013
© 2013 John Wiley & Sons Ltd
Volume 18, Issue 6, pages 423–432, December 2013
How to Cite
Lagunes-Servin, H., Torres, J., Maldonado-Bernal, C., Pérez-Rodríguez, M., Huerta-Yépez, S., Madrazo de la Garza, A., Muñoz-Pérez, L., Flores-Luna, L., Ramón-García, G. and Camorlinga-Ponce, M. (2013), Toll-Like Receptors and Cytokines are Upregulated during Helicobacter pylori Infection in Children. Helicobacter, 18: 423–432. doi: 10.1111/hel.12067
- Issue published online: 23 OCT 2013
- Article first published online: 22 JUL 2013
- IMSS. Grant Numbers: IMSS-2004/009, IMSS-2005/1/110
- CONACYT. Grant Number: 174761
- H. pylori ;
- TLR ;
- gastric mucosa
Background and Aim
Helicobacter pylori infection is mainly acquired during childhood, and establishes a chronic infection that may lead to peptic ulcer or gastric cancer during adulthood. Toll-like receptors (TLRs) are expressed by distinct cell types throughout the gastrointestinal tract, and play an important role in regulation of the innate immune response. Few works have addressed TLRs expression in gastric epithelia of adults, and scarce studies have done it in children. The aim of this work was to analyze the expression of TLR2, TLR4, TLR5, TLR9, and IL-8, IL-10 and TNF-α in the gastric mucosa of children with and without H. pylori infection.
Gastric biopsies were collected by endoscopy from 50 children with recurrent abdominal pain, 25 with H. pylori infection and 25 without infection. In the gastric biopsies the expression of TLRs and cytokines was studied by immunohistochemistry, and the degree of mucosal inflammation was determined using the Sydney system.
We found that H. pylori infection was associated with a significant increased expression of TLRs 2, 4, 5 and 9, although expression varied between surface epithelia and glands. Epithelial cells expressing IL-8, IL-10 and TNF-α were increased in gastric mucosa of children with H. pylori infection.
This study shows the gastric epithelia of children respond to H. pylori infection by increasing the expression of TLR2, TLR4, TLR5, TLR9 and the cytokines IL-8, IL-10 and TNF-α.