Organization, barrier function and antimicrobial lipids of the oral mucosa

Authors


Correspondence to: Philip Wertz, University of Iowa, N450 DSB, Iowa City, Iowa 52242, UK. Tel.: +319-335-7409; fax: +319-335-8895; e-mail: Philip-wertz@uiowa.edu

Synopsis

As one moves from the skin across the vermilion region of the lip and into the oral cavity, the oral mucosa is encountered. The oral mucosa consists of connective tissue known as the lamina propria covered by a stratified squamous epithelium. In the regions of the hard palate and gingiva, the epithelium is keratinized like the epidermis. In the buccal region, the floor of the mouth and the underside of the tongue, the epithelium is non-keratinized. The epithelium on the dorsum of the tongue is a specialized epithelium, but can be approximated as a mosaic of keratinized and non-keratinized epithelia. The non-keratinized epithelial regions do not produce a stratum corneum. Nuclei with intact DNA are retained in the superficial cells. In all regions, the outer portions of the epithelium provide a protective permeability barrier, which varies regionally. Antimicrobial lipids at the surfaces of the oral mucosa are an integral part of innate immunity.

Résumé

Quand on se déplace de la peau vers la région du vermillon de la lèvre et puis vers la cavité buccale, on rencontre la muqueuse buccale. La muqueuse buccale est constituée de tissu conjonctif appelé lamina propria, recouverte par un épithélium pavimenteux stratifié. Dans les régions de la voûte du palais et de la gencive, l'épithélium est kératinisé comme l'épiderme. Dans la région buccale, le plancher de la bouche et de la face inférieure de la langue, l'épithélium n'est pas kératinisé. L'épithélium de la face dorsale de la langue est un épithélium spécialisé, mais peut être vu comme un mosaïque d'épithélium kératinisé et non-kératinisé. Les régions non-kératinisées épithéliales ne produisent pas de stratum corneum. Les noyaux avec de l'ADN intact sont retenus dans les cellules superficielles. Dans toutes les régions, les parties externes de l'épithélium fournissent une barrière de perméabilité de protection, qui varie selon les régions. Des lipides antimicrobiens à la surface de la muqueuse buccale sont une partie intégrante de l'immunité innée.

Introduction

The purpose of this review was to point out some of the similarities and differences between skin and oral mucosa, with special emphasis on epidermis and oral epithelia from different regions of the oral cavity. Within the oral cavity, some mucosal regions are covered by a keratinized epithelium resembling epidermis, whereas other regions are lined by a non-keratinizing epithelium [1-3]. Lipids in the outer portion of oral epithelium determine the permeability barrier function, and certain lipids provide an antimicrobial barrier at the epithelial surfaces [4-6]. Emphasis will be placed on epithelial biology, barrier function and antimicrobial lipids.

Lip

The lip is a specialized region that represents the transition from the skin to the oral mucosa. The line where the skin surrounding the red external lip meets the surrounding facial skin is the vermillion border of the lip. The exterior vermillion zone of the lip has a thin keratinized epithelium [1]. The underlying connective tissue has a rich blood supply, which contributes to the red colouration [7]. The projected area of the corneocytes on the vermillion zone of the lip is slightly larger than that of corneocytes from the cheek, and the TEWL through the lip is greater than TEWL through the cheek [8]. Surprisingly, the hydration of the external vermillion zone of the lip is greater than the hydration of the inner lip mucosa [9]. Within the connective tissue along the vermillion border of the lip are sebaceous follicles, which are sebaceous glands without associated terminal hairs. The composition of the lipid mixture produced by these sebaceous follicles is the same as that produced by the major sebaceous glands of the skin [10]. The composition of sebum in the lumen of the gland is 57% triglycerides, 25% wax monoesters, 15% squalene, 2% cholesterol esters and 1% cholesterol [11]. As sebum flows to and over the skin surface, the triglycerides undergo partial hydrolysis to release fatty acids, some of which are antimicrobial [3]. The most notable antimicrobial fatty acids are lauric acid (C12:0) and sapienic acid (C16:1Δ6). Sebaceous follicles are not only found in the vermillion border of the lip and in the oral mucosa, they encircle every orifice of the human body [3]. This distribution strongly suggests a protective function. As one progresses from the external vermillion zone of the lip into the oral cavity, there is a transition from keratinized to non-keratinized epithelium.

Lamina propria

The lamina propria is the mucosal connective tissue [12]. In general, the lamina propria consists of two compartments: the papillary layer and the reticular layer. The papillary layer is immediately beneath the epithelium. It contains loosely organized collagen fibres and capillary loops. Nerve endings are found in the papillary layer or sometimes extend into the epithelium. The lower reticular layer contains bundles of collagen fibres arranged parallel to the plane of the mucosa. There are also elastic fibres within the lamina propria. The reticular layer contains sebaceous follicles and major and minor salivary glands. The major salivary glands are the parotid, parietal and sublingual glands, and there is usually one or several lobes that undergo sebaceous differentiation [13-15]. The minor salivary glands and sebaceous follicles are numerous and are found throughout the oral mucosa. These sebaceous follicles, if enlarged, are sometimes called Fordyce spots or granules. Fordyce spots on the vermillion border of the lip are sometimes treated for cosmetic reasons [16]. The association of sebaceous lobes with major salivary glands and the ubiquitous presence of sebaceous follicles in oral mucosa explain why the lipids at the mucosal surfaces and in saliva have the same composition as lipids at the skin surface [17]. The antimicrobial fatty acids, lauric acid and sapienic acid, likely contribute to control of microbial growth within the oral cavity [4, 18].

In the regions of the hard palate and gingival, the lamina propria is anchored to the periostium of the underlying bone. This arrangement allows these regions to withstand the shearing forces associated with chewing food [3]. In the non-keratinizing regions, there is a submucosa beneath the reticular layer that attaches to muscle. This, in combination with some regional differences in collagen and elastin, allows these regions of the oral mucosa to be more flexible, which is essential for speech [3]. The lamina propria contains fibroblasts that make collagen. There are also mast cells, macrophages and other cellular components of the immune system [19].

Keratinized oral epithelium

Most of the cells in the keratinizing oral epithelia are keratinocytes. Keratin gene expression varies as a function of differentiation [20]. Genes are expressed pair-wise, so that a type I, or acidic, keratin is paired with a type II, or basic/neutral, keratin [20, 21]. In the basal cells of the keratinizing oral epithelia, keratin 5 and keratin 14 are expressed. As keratinocytes undergo differentiation, production of keratins 5 and 14 stops and keratins 6 and 16 are co-expressed followed by keratins 1 and 10. Approximately, 85 per cent of the protein in the stratum corneum is keratin [22].

In addition to keratins, differentiating keratinocytes in the regions of the gingiva and hard palate accumulate proteins in the form of keratohyalin granules [20]. One of these proteins is profilaggrin [23]. This is a high molecular weight, histidine-rich highly phosphorylated protein. At the end of the differentiation programme, profilaggrin is dephosphorylated and proteolytically cleaved to produce 10 to 12 filaggrin units [24]. Filaggrin causes aggregation of the keratin filaments into dense bundles of keratin that lie parallel to the plane of the epithelium. This collapse of the cytoskeletal network is associated with the extreme flattening of the cells in the stratum corneum. After the aggregation of the keratin, filaggrin is broken down into its component amino acids [25]. This results in an extremely high osmolarity (approximately 2 M) within the cornified cells. In addition to amino acids, the cornified cells also contain a significant amount of lactate, a product of anaerobic glycolysis that contributes to the high osmotic strength and is important in maintaining a low pH [26]. This high osmotic strength of the interior of the corneocytes essentially removes all free water out of the intercellular spaces of the stratum corneum.

Just prior to the time the cytoskeletal system collapses, the nucleus, mitochondria and other internal organelles are degraded [27]. This results in release of ionic calcium that had been sequestered by the membrane system [28].

In addition to accumulating proteins, differentiating keratinocytes in the keratinizing regions accumulate lipids [29]. Much of this lipid accumulates in the form of lamellar granules [27]. In transmission electron micrographs, these organelles appear round to ovoid in shape and are about 0.2 μm in diameter [30]. They have a unit bounding membrane surrounding one or sometimes several stacks of internal lamellae. In the uppermost granular layer, the bounding membrane of the lamellar granule fuses into the cell plasma membrane and the contents are extruded into the intercellular space. The initially extruded lipid is largely a mixture of phospholipids, glucosylceramides and cholesterol. Hydrolytic enzymes, also delivered by the lamellar granules [31-33], act on the initially extruded phospholipids and glucosylceramides to produce ceramides and fatty acids. In contrast to the epidermis, small amounts of phospholipid and glucosylceramide pass into the stratum corneum [34].

At about the same time the cytoskeletal system collapses, the cell plasma membrane is replaced by a cornified envelope [20, 35]. This is a thick band of protein, some of which is derived largely from involucrin from the keratohyalin granules. This band of protein is held together by isopeptide linkages formed through the action of a transglutaminase and by disulphide linkages [27, 35]. The outer surface of this polymerized protein contains only small amounts of covalently bound lipid compared with what is found in epidermal stratum corneum [36]. The main components of this covalently bound lipid are ω-hydroxyceramides [37, 38]. The ω-hydroxyacid components of these ceramides are 30- through 34-carbons in length. These hydroxyceramides are ester-linked to acidic side chains on the surface of the protein envelope through ester-linkages involving the ω-hydroxyl group [39].

The end product of the keratinization product is the stratum corneum [27]. This consists of flat, hexagonal cells embedded in a lipid matrix. The cells are filled with keratin and a high concentration of amino acids and other low molecular weight substances and are bounded by a thick band of polymerized protein with covalently bound lipid on the outer surface. The lipids filling the intercellular spaces throughout the stratum corneum consist mostly of ceramides, cholesterol, fatty acids and cholesterol sulphate. There are small proportions of phospholipids, glucosylceramides and cholesterol esters [34]. The stratum corneum provides a permeability barrier, but it is not as impermeable as the barrier of the skin [40].

In epidermal stratum corneum, the free fatty acids as well as the amide-linked fatty acids in the ceramides are predominantly long (20–28 carbons) and saturated [41, 42]. The ester-linked fatty acid in the acylceramide, EOS, is linoleic acid. In contrast, in oral stratum corneum, the amide-linked ω-hydroxyacids cover the same range of chain lengths, but they are a mixture of saturated and monounsaturated species [43]. Also, the major fatty acids ester-linked to the acylceramide are palmitic, stearic and eicosanoic acids. Linoleate is only 9% of the total.

In epidermal and oral stratum corneum, ceramides, fatty acids and cholesterol are the primary lipids that determine the permeability barriers. The epidermal fatty acids and ceramides are rod or cylindrical in shape. This is ideal for the formation of highly ordered, and thereby highly impermeable, membrane domains. The cholesterol would provide a degree of flexibility to avoid brittleness. In oral stratum corneum, some of the fatty acids and ceramides, by virtue of the presence of cis double bonds, are no longer cylindrical in shape. In addition, oral stratum corneum contains small amounts of phospholipids and glucosylceramides that are not present in epidermal stratum corneum. These lipids are more conical in shape and suited for the formation of fluid membranes. These geometric factors contribute to the greater permeability of the keratinized oral regions compared with skin.

Non-keratinized epithelium

The inside of the lips and cheeks, the floor of the mouth and the underside of the tongue are covered by a non-keratinized epithelium [6]. Again, most of the cells in these epithelial regions are keratinocytes. In contrast to the keratinizing epithelia, internal cellular organelles are not degraded in non-keratinizing epithelia. Intact nuclei with high molecular weight DNA survive to the surface layers. The basal keratinocytes in these regions express K5 and K14/K19 [20]. Other keratinocytes expressed at later times during differentiation include K4, K13 and low levels of K1 and K10. Keratohyalin granules are not produced in the non-keratinizing epithelia.

The non-keratinizing epithelia do not produce lamellar granules, but they do produce two similar secretory organelles: a cored granule [44] and a lamellate granule [34]. Both of these organelles are about the same size and shape (spherical and approximately 0.2-μm diameter) as the lamellar granules. They secrete their contents into the intercellular spaces about 2/3 of the way from the basal layer to the surface. This corresponds to a change in permeability, so the outer third of the epithelium provides the permeability barrier [3]. This superficial barrier, although it contains internal organelles including mitochondria, is metabolically inactive. The barrier function is less effective than that of the keratinized regions [40].

The superficial layers of the non-keratinizing oral epithelia contain abundant phospholipids, which reflect the fact that mitochondria, nuclei, plasma membranes, etc. are not degraded in these epithelial regions. These lipids probably have nothing to do with barrier function. There are small amounts of ceramides in addition to saturated fatty acids and cholesterol. In fact, a strong correlation (r2 = 0.9) has been found for ceramide content and barrier function across all epithelial regions [34].

Other cell types

In addition to keratinocytes, the oral epithelia contain three other cell types [19]. The basal layer includes Merkel cells, which are usually adjacent to nerve endings. These cells are involved in sensory function. The suprabasal layer contains two populations of dendritic cells. The Langerhans cells are antigen-presenting cells and part of the immune system. Melanocytes are pigment-producing cells.

Conclusion

The pattern of epithelial terminal differentiation varies regionally within the oral cavity. Epithelial thickness, structural proteins and permeability barrier function vary between skin and oral mucosa and among different oral mucosal regions. Certain lipids, mainly ceramides saturated fatty acids and cholesterol, determine the permeability barrier functions of skin and different regions of oral mucosa. Other lipids (sphingosine, sapienic acid and lauric acid) provide for antimicrobial barriers.

Acknowledgements

This work was supported by funds from R01 DE014390 and RO1 DEO18032 from the National Institute of Dental and Craniofacial Research, National Institutes of Health.

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