Effect of Simvastatin on kinetics of Osteoprotegrin/receptor activator nuclear kappa B Ligand mRNA expression in periapical lesions
Article first published online: 18 APR 2013
© 2013 International Endodontic Journal. Published by John Wiley & Sons Ltd
International Endodontic Journal
Volume 46, Issue 11, pages 1077–1082, November 2013
How to Cite
Effect of Simvastatin on kinetics of Osteoprotegrin/receptor activator nuclear kappa B Ligand mRNA expression in periapical lesions. International Endodontic Journal, 46, 1077–1082, 2013., .
- Issue published online: 4 OCT 2013
- Article first published online: 18 APR 2013
- Accepted manuscript online: 14 MAR 2013 06:49AM EST
- Manuscript Accepted: 19 FEB 2013
- Manuscript Received: 6 JAN 2012
- Isfahan University of Medical Sciences. Grant Number: 389217
- periapical region;
- RANKL ;
To evaluate the therapeutic effect of Simvastatin on apical periodontitis in rats by assessing the osteoblast production of receptor activator of nuclear factor–Kappa B Ligand (RANKL) and Osteoprotegerin (OPG) cytokines that are essential for bone resorption.
Twenty-five healthy 8- to 10-week-old male rats were selected. Periapical lesions were induced by exposure of pulps through the occlusal surfaces of mandibular first molars. The pulps were extirpated and canals contaminated with saliva. The teeth were filled temporarily to make animal feeding feasible. The experimental group (n = 12) was placed on 20 mg−1 kg−1 day−1 of Simvastatin for 10 days (3 days before and 7 days after pulp exposure) and the control group (n = 12) received a placebo. After 1, 2 and 4 weeks, the animals were sacrificed and mRNA expression of OPG/RANKL was evaluated by real-time PCR. One rat was sacrificed without any interventions (negative control) to determine the baseline expression of the mediators involved. Data were analysed by one-way anova.
Simvastatin significantly (P < 0.05) enhanced the expression of OPG and reduced the mRNA expression of RANKL in the experimental group compared with the control group.
Oral administration of Simvastatin attenuated RANKL expression and accelerated OPG expression in induced rat periapical lesions.