Conflicts of interest: None.
Skin barrier impairment correlates with cutaneous Staphylococcus aureus colonization and sensitization to skin-associated microbial antigens in adult patients with atopic dermatitis
Version of Record online: 24 JUL 2013
© 2013 The International Society of Dermatology
International Journal of Dermatology
Volume 53, Issue 1, pages 27–33, January 2014
How to Cite
Jinnestål, C. L., Belfrage, E., Bäck, O., Schmidtchen, A. and Sonesson, A. (2014), Skin barrier impairment correlates with cutaneous Staphylococcus aureus colonization and sensitization to skin-associated microbial antigens in adult patients with atopic dermatitis. International Journal of Dermatology, 53: 27–33. doi: 10.1111/ijd.12198
Funding: This work was supported by grants from the Swedish Government Funds for Clinical Research (ALF), the Welander and Finsen Research Foundations, Alfred Osterlund research foundation, and the Eva and Oscar Ahrens Research Foundation.
- Issue online: 18 DEC 2013
- Version of Record online: 24 JUL 2013
- Swedish Government Funds for Clinical Research
- Welander and Finsen Research Foundations
- Alfred Österlund research foundation
- Eva and Oscar Ahréns Research Foundation
Atopic dermatitis (AD) is a chronic inflammatory skin disease. The pathogenesis of AD involves skin barrier defects and dysregulation of innate and adaptive immunity. Some environmental factors such as stress, infections, and allergens are associated with aggravation of AD. The aim of the study was to investigate the relationship between skin barrier function, skin colonization of Staphylococcus aureus, and sensitization to antigens of skin-associated microorganisms in adult patients with AD.
Thirty adult patients with AD and 10 controls were recruited. Eczema severity was assessed, and transepidermal water loss (TEWL) was measured. Bacterial samples were taken from the skin using a swab technique for qualitative identification of S. aureus and a contact agar disc method for quantitative assessment. Immunological analyses of specific IgE to staphylococcal enterotoxins and yeasts as well as total serum IgE levels, were performed.
TEWL was significantly higher among S. aureus-positive patients in comparison to S. aureus-negative patients with AD (P < 0.05). TEWL increased with increasing bacterial load (P = 0.018). In the group of patients sensitized to all three of the investigated skin-associated microorganisms (S. aureus, Malassezia, and Candida), an increased TEWL was observed, in comparison to patients sensitized to none, or one or two (P = 0.026).
In adult patients with AD, a disrupted skin barrier promotes skin colonization by microbes, such as S. aureus. Heavy microbial colonization may facilitate skin penetration of microbial antigens leading to subsequent IgE sensitization. These results illustrate the importance of skin-associated microbial colonization and sensitization to microbial-derived allergens in eczema pathogenesis.