Cytogenetic biomonitoring in oral leukoplakia patients with mild dysplasia

Authors

  • Mariano Sánchez-Siles DDS, PhD,

    Corresponding author
    1. Department of Oral Medicine, University of Murcia, Murcia, Spain
    2. Department of Dentistry, University of Murcia, Murcia, Spain
    • Correspondence

      Mariano Sánchez-Siles, dds, phd,

      Professor of Oral Medicine, University of Murcia

      Medicina Oral

      Hospital Morales Meseguer

      Avda

      Marqués de los Velez s/n

      30008 Murcia

      Spain

      E-mail: marianosasi@um.es

    Search for more papers by this author
  • Fabio Camacho-Alonso DDS, PhD,

    1. Department of Oral Medicine, University of Murcia, Murcia, Spain
    2. Department of Dentistry, University of Murcia, Murcia, Spain
    Search for more papers by this author
  • Irene Ros-Llor DDS, PhD,

    1. Department of Oral Medicine, University of Murcia, Murcia, Spain
    2. Department of Dentistry, University of Murcia, Murcia, Spain
    Search for more papers by this author
  • Pia López-Jornet DDS, MD, PhD

    1. Department of Oral Medicine, University of Murcia, Murcia, Spain
    2. Department of Dentistry, University of Murcia, Murcia, Spain
    Search for more papers by this author

Abstract

Objective

A study is made of DNA damage and apoptosis in a group of patients with oral leukoplakia (OL) with mild dysplasia.

Materials and methods

The study comprised 30 patients with a clinicopathological diagnosis of OL with mild dysplasia and 30 controls. Both samples were similar in terms of age and gender distribution. Brush samples of lesion epithelial cells were collected, followed by cell centrifugation, preparation of the slides, fixation and staining, and analysis under the fluorescent light microscope. The exfoliated cells were examined to detect micronuclei (MN), nuclear buds, binucleated cells, condensed chromatin, pyknosis, and cells with karyorrhexis and karyolysis.

Results

The patients with OL with mild dysplasia showed a greater frequency of MN (< 0.001), nuclear buds (= 0.018), and binucleated cells (= 0.008).

Conclusions

Cytogenetic biomonitoring is a simple and scantly invasive technique allowing clinicians to assess DNA damage and apoptosis in patients with OL.

Clinical relevance

Oral cancer should be detected and controlled in its precancerous stages in order to increase survival rates. Leukoplakia lesions must be biomonitorized periodically. Biomonitorization offers sensibility, no morbidity, speed, and low cost.

Ancillary