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Physicochemical and functional properties of chitosans affected by storage periods of crab leg shell


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Effects of storage period (0, 1, 2, 3 and 4 months) of crab leg shell at room temperature on selected physicochemical and functional properties of E- and G-chitosans, respectively, prepared from entire (E) and ground (G) shells were evaluated. Increased storage period of crab shell generally increased degree of deacetylation (DD) and viscosity, but decreased DPPH radical scavenging activity of both chitosans. Water-binding (WBC) and fat-binding (FBC) capacities of E-chitosan were not affected by storage period of crab shell; however, those of G-chitosan significantly increased when crab shell was stored for more than 1 month. Dye-binding capacity (DBC) of both chitosans decreased when crab shell was stored for 1 month, but further decrease was not observed with increased storage period to 4 months. Prepared with 4-month stored crab shell, E-chitosan exhibited comparable viscosity, colour whiteness index, DPPH radical scavenging activity and DBC, but lower DD, WBC and FBC than G-chitosan.