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Characteristics of Aspergillus niger xylanases produced on rice husk and wheat bran in submerged culture and solid-state fermentation for an applicability proposal

Authors

  • Isabel Membrillo Venegas,

    1. División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, Ecatepec, México
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    • Both authors contributed equally to this work.
  • Janeth Fuentes-Hernández,

    1. División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, Ecatepec, México
    2. Universidad Tecnológica de Tecámac, Tecámac, México
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    • Both authors contributed equally to this work.
  • Mayola García-Rivero,

    1. División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, Ecatepec, México
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  • Aurora Martínez-Trujillo

    Corresponding author
    • División de Ingeniería Química y Bioquímica, Tecnológico de Estudios Superiores de Ecatepec, Ecatepec, México
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Correspondent: E-mail: amartinezt@tese.edu.mx

Summary

Xylanolytic rich filtrates were obtained by A. niger sp in both submerged and solid-state culture using rice husk or wheat bran as the only carbon source. Filtrates obtained on rice husk showed the highest activities (~6500 and 5200 U g−1, respectively). Independent of carbon source, these filtrates were very stable in an acidic pH range (4–7) and mild temperatures, with high half-life time values (more than 7 h at 50 °C) in the corresponding inactivation kinetic models. Also the effect of different metallic ions and denaturing substances was verified finding that these enzymes are not metaloproteins, and metals as Hg2+ and Pb2+ caused the greatest loss of xylanolytic activity (not higher than 30%). Xylanases produced by this A. niger strain showed important features that make them potential candidates for applications on human and livestock food industries.

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